{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,11]],"date-time":"2025-05-11T00:00:35Z","timestamp":1746921635031,"version":"3.40.4"},"reference-count":18,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med Princ Pract"],"published-print":{"date-parts":[[2014]]},"abstract":"<b><i>Objective:<\/i><\/b> To evaluate the impact of brief and sequential exposure to nystatin on the germ tube formation and cell surface hydrophobicity of oral isolates of <i>Candida albicans <\/i>obtained from patients infected with human immunodeficiency virus (HIV). <b><i>Materials and Methods:<\/i><\/b> After determining the minimum inhibitory concentration of nystatin, 10 oral isolates of <i>C. albicans<\/i> from 10 different HIV-infected patients were briefly (1 h) and sequentially (10 days) exposed to subtherapeutic concentrations of nystatin. Following a subsequent drug removal, the germ tube formation and cell surface hydrophobicity of these isolates were determined via a germ tube induction assay and an aqueous hydrocarbon assay, respectively. The data obtained from these assays for the control (unexposed to nystatin) and nystatin-exposed isolates were analyzed using Student's t tests. <b><i>Results:<\/i><\/b> The mean percentage reduction in the germ tube formation and cell surface hydrophobicity of the nystatin-exposed isolates compared to the controls was 30.12 \u00b1 1.99 (p < 0.001) and 29.65 \u00b1 2.33 (p < 0.001), respectively. <b><i>Conclusion:<\/i><\/b> These data elucidate the possible pharmacodynamic mechanisms by which nystatin might operate in vivo in the modulation of candidal virulence.<\/jats:p>","DOI":"10.1159\/000362369","type":"journal-article","created":{"date-parts":[[2014,4,30]],"date-time":"2014-04-30T06:38:55Z","timestamp":1398839935000},"page":"307-312","source":"Crossref","is-referenced-by-count":8,"title":["Impact of Brief and Sequential Exposure to Nystatin on the Germ Tube Formation and Cell Surface Hydrophobicity of Oral <b><i>Candida Albicans<\/i><\/b> Isolates from Human Immunodeficiency Virus-Infected Patients"],"prefix":"10.1159","volume":"23","author":[{"given":"Arjuna N.B.","family":"Ellepola","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshman P.","family":"Samaranayake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,4,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511400912"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/144.6.565"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(97)00026-5"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb06008.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1080\/02681219880000591"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1998.tb00220.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1999.tb02007.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159\/000339080"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(98)00064-8"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1998.tb01944.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159\/000332569"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2012.02203.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159\/000345641"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-77-2-417"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(94)90012-4"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-42-5-362"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12102"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2011.11.001"}],"container-title":["Medical Principles and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/362369","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/362369","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/362369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:38:36Z","timestamp":1745429916000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000362369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":18,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,7,1]]},"published-print":{"date-parts":[[2014,7,18]]}},"URL":"https:\/\/doi.org\/10.1159\/000362369","archive":["Portico"],"relation":{},"ISSN":["1011-7571","1423-0151"],"issn-type":[{"type":"print","value":"1011-7571"},{"type":"electronic","value":"1423-0151"}],"subject":[],"published":{"date-parts":[[2014]]}}}