{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T20:21:59Z","timestamp":1789762919102,"version":"4.0.1"},"reference-count":67,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background\/Aims:&lt;\/i&gt;&lt;\/b&gt; To investigate the inhibitory effect of green tea extract, epigallocatechin gallate (EGCG), on wild-type human leiomyoma (WT-HuLM) cells and its potential action via catechol-&lt;i&gt;o&lt;\/i&gt;-methyltransferase (COMT) activity. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Cell proliferation of WT-HuLM and COMT gene-silenced HuLM (COMT-shRNA-HuLM) cells treated with 0 or 100 \u00b5&lt;smlcap&gt;M&lt;\/smlcap&gt; EGCG for 7 days was measured using the MTT method. Total RNA and protein were extracted from cells treated with 0 or 100 \u00b5&lt;smlcap&gt;M&lt;\/smlcap&gt; of EGCG for 48 h. Gene expression profiling was performed using Human Signal Transduction PathwayFinder. Proliferation cell nuclear antigen (PCNA), cyclin-dependent kinase 4 (Cdk4) and COMT protein levels were detected by Western blot analyses. COMT enzyme activity was evaluated by HPLC. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; EGCG-treated WT-HuLM cells showed significantly decreased COMT expression (p &lt; 0.001) and enzyme activity (p &lt; 0.05) compared to untreated WT-HuLM cells, while COMT-shRNA-HuLM cells showed no significant change. At 100 \u03bc&lt;smlcap&gt;M&lt;\/smlcap&gt; of EGCG, survival of WT-HuLM cells was significantly lower (p &lt; 0.05) compared to COMT-shRNA-HuLM cells. EGCG treatment modulated multiple signaling pathways in WT-HuLM compared to untreated control, while changes were minimal or reversed in COMT-shRNA-HuLM cells. EGCG significantly decreased PCNA, Cdk4 and soluble COMT protein levels (p &lt; 0.001) in WT-HuLM, but not in COMT-shRNA-HuLM cells. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; The antiproliferative and gene-modulating effects of EGCG on HuLM cells are mediated, at least partially, via its effect on COMT expression and enzyme activity.<\/jats:p>","DOI":"10.1159\/000363410","type":"journal-article","created":{"date-parts":[[2014,6,18]],"date-time":"2014-06-18T08:38:12Z","timestamp":1403080692000},"page":"109-118","source":"Crossref","is-referenced-by-count":28,"title":["Green Tea Extract Inhibition of Human Leiomyoma Cell Proliferation Is Mediated via 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