{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T01:15:51Z","timestamp":1760577351981,"version":"3.40.4"},"reference-count":28,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>B chromosomes in the grasshopper &lt;i&gt;Eyprepocnemis plorans&lt;\/i&gt; are considered to be mitotically stable, because all meiotic (primary spermatocytes and oocytes) or mitotic (embryos, ovarioles, and gastric caecum) cells analyzed within the same individual show the same B chromosome number. Nothing is known, however, about body parts with somatic tissues with no mitotic activity in adult individuals, constituting the immense majority of their body. Therefore, we investigated whether B chromosomes are present in 8 non-mitotically active somatic body parts from both sexes in addition to ovarioles and testes by PCR analysis of 2 B-specific molecular markers. We also elucidated the number of B chromosomes that an individual carried through quantifying the B-located rDNA copy number by qPCR. Our results indicated the amplification of both B-specific markers in all analyzed body parts. However, we found high variation between males for the estimated number of rDNA units in the B chromosomes. These results demonstrate the presence of B chromosomes in all body parts from the same individual and suggest a high variation in the rDNA content of the B chromosomes carried by different individuals from the same population, presumably due to unequal crossovers during meiosis.<\/jats:p>","DOI":"10.1159\/000365797","type":"journal-article","created":{"date-parts":[[2014,8,18]],"date-time":"2014-08-18T07:04:45Z","timestamp":1408345485000},"page":"268-274","source":"Crossref","is-referenced-by-count":4,"title":["B Chromosomes in the Grasshopper &lt;b&gt;&lt;i&gt;Eyprepocnemis plorans&lt;\/i&gt;&lt;\/b&gt; Are Present in All Body Parts Analyzed and Show Extensive Variation for rDNA Copy Number"],"prefix":"10.1159","volume":"143","author":[{"given":"Mercedes","family":"Ruiz-Est\u00e9vez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josefa","family":"Cabrero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Pedro M.","family":"Camacho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Dolores","family":"L\u00f3pez-Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,8,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000079302"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6885960"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012301463-4\/50006-1"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1080\/00087114.1983.10797651"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/BF00286298"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.2144\/000112750"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00281927"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2307\/2409449"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293310"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/BF00326973"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.1995.tb03079.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.1971.76"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/BF01553487"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.1995.148"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.1972.97"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.110.122713"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007\/BF00326108"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.1995.24"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159\/000079313"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1007\/BF00015882"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-192-2:365"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036600"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007\/s10709-013-9733-6"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-8-47"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-013-9606-y"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-5223.1994.00157.x"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.2013.135"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.2307\/2410945"}],"container-title":["Cytogenetic and Genome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/365797","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/365797","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:02:01Z","timestamp":1745431321000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000365797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":28,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,10,1]]},"published-print":{"date-parts":[[2014,10,21]]}},"URL":"https:\/\/doi.org\/10.1159\/000365797","archive":["Portico"],"relation":{},"ISSN":["1424-8581","1424-859X"],"issn-type":[{"type":"print","value":"1424-8581"},{"type":"electronic","value":"1424-859X"}],"subject":[],"published":{"date-parts":[[2014]]}}}