{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:29:20Z","timestamp":1747193360185,"version":"3.40.5"},"reference-count":33,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Res Paediatr"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; Aggregation of growth hormone (GH) required for its proper storage in granules is facilitated by zinc (Zn&lt;sup&gt;2+&lt;\/sup&gt;) transported by specific zinc transporters in and out of the regulated secretory pathway. &lt;i&gt;Slc30a5&lt;\/i&gt; (ZnT5) was reported to have the highest gene expression among all zinc transporters in primary mouse pituitary cells while &lt;i&gt;ZnT5&lt;\/i&gt;-null mice presented with abnormal bone development and impaired growth compared to wild-type counterparts. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; In vitro studies performed in GH3 cells, a rat pituitary cell line that endogenously produces rat GH (rGH), included analysis of: cytoplasmic Zn&lt;sup&gt;2+&lt;\/sup&gt; pool changes after altering rSlc30a5 expression (luciferase assay), rZnT5 association with different compartments of the regulated secretory pathway (confocal microscopy), and the rGH secretion after rSlc30a5 knock-down (Western blot). &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Confocal microscopy demonstrated high co-localization of rZnT5 with ER and Golgi (early secretory pathway) while siRNA-mediated knock-down of rSlc30a5 gene expression led to a significant reduction in rGH secretion. Furthermore, altered expression of rSlc30a5 (knock-down\/overexpression) evoked changes in the cytoplasmic Zn&lt;sup&gt;2+&lt;\/sup&gt; pool indicating its important role in mediating Zn&lt;sup&gt;2+&lt;\/sup&gt; influx into intracellular compartments of the regulated secretory pathway. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Taken together, these results suggest that ZnT5 might play an important role in regulated GH secretion that is much greater than previously anticipated.<\/jats:p>","DOI":"10.1159\/000365924","type":"journal-article","created":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T07:51:33Z","timestamp":1410249093000},"page":"245-251","source":"Crossref","is-referenced-by-count":5,"title":["Alteration of ZnT5-Mediated Zinc Import into the Early Secretory Pathway Affects the Secretion of Growth Hormone from Rat Pituitary 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