{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T18:55:12Z","timestamp":1789239312582,"version":"build-2803163510"},"reference-count":35,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T00:00:00Z","timestamp":1411430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T00:00:00Z","timestamp":1411430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>IRF8 (interferon-regulatory factor-8) plays a critical role in regulating myeloid cell differentiation. However, the role of this transcription factor in the development of Ly6C+ inflammatory monocytes and their migration to the infected brain has not been examined. We have previously shown that West Nile virus (WNV) infection of wild-type (WT) mice triggers a significant increase in numbers of Ly6C+ monocytes in the bone marrow. These cells traffic via the blood to the infected brain, where they give rise to proinflammatory macrophages. Here, we show that WNV-infected IRF8-deficient (IRF8-\/-) mice had significantly reduced numbers of Ly6C+ monocytes in the periphery, with few of these cells found in the blood. Furthermore, low numbers of inflammatory monocyte-derived macrophages were observed in the brains of IRF8-\/- mice throughout infection. Adoptive transfer of IRF8-\/- Ly6C+ monocytes demonstrated that these cells were intrinsically unable to traffic to the inflamed brain. Low expression of the chemokine receptor CCR2 and integrin VLA-4 by IRF8-\/- monocytes likely contributed to this defect, as the interactions between these proteins and their ligands are critical for monocyte egress and migration to inflammatory foci. These data highlight a critical role for IRF8 in inflammatory monocyte differentiation and migration during WNV infection.<\/jats:p>","DOI":"10.1159\/000365972","type":"journal-article","created":{"date-parts":[[2014,9,22]],"date-time":"2014-09-22T02:42:31Z","timestamp":1411353751000},"page":"102-112","source":"Crossref","is-referenced-by-count":25,"title":["Defective Inflammatory Monocyte Development in IRF8-Deficient Mice Abrogates Migration to the West Nile Virus-Infected Brain"],"prefix":"10.1159","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6260-7372","authenticated-orcid":false,"given":"Rachael L.","family":"Terry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine","family":"Deffrasnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Getts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Minten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caryn","family":"van Vreden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Ashhurst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghann T.","family":"Getts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui Dan Vicki","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain L.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3877-9772","authenticated-orcid":false,"given":"Nicholas J.C.","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2014,9,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-168575"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-7-92"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080421"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6209-10.2011"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp144"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-246"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3007563"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2008.19"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178331"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.221127"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132557"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2063"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1100066"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-01-129049"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-07-367706"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81348-3"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-06-364976"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091627"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.3.1131"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222798110"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-437863"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.061986"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20111009"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175202"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1038\/ni1309"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003003"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002682"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-02-0569"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04798.x"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1007\/BF01718310"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1038\/nri1375"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040760"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.3.1283"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.13.6898"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62636"}],"container-title":["Journal of Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/365972","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/365972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T13:34:00Z","timestamp":1745415240000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000365972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,23]]},"references-count":35,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,12,1]]},"published-print":{"date-parts":[[2014,12,11]]}},"URL":"https:\/\/doi.org\/10.1159\/000365972","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5126932","asserted-by":"subject"}]},"ISSN":["1662-811X","1662-8128"],"issn-type":[{"value":"1662-811X","type":"print"},{"value":"1662-8128","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,23]]}}}