{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T04:41:57Z","timestamp":1770439317159,"version":"3.49.0"},"reference-count":22,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Background: This paper reports the case of an egg-allergic pediatric patient who, once desensitized to egg following a successful rush oral immunotherapy protocol, could also tolerate Lizipaina\u00ae, a drug containing lysozyme (LYS) and papain, which had previously caused him a severe allergic reaction. Because the LYS amount that elicited the anaphylactic reaction (5 mg) was much lower than that tolerated during a double-blind placebo-controlled food challenge (corresponding to approximately 60 mg of LYS), the possibility that the presence of papain could increase the allergenic potential of LYS was investigated. Methods: Lizipaina, LYS and LYS hydrolyzed with papain were analyzed by SDS-PAGE under reducing and nonreducing conditions, and Western blotting of sera from egg-allergic patients was performed in order to detect IgE-binding fragments. Finally, sequence identification of the IgE-reactive bands was carried out by MALDI-TOF\/TOF. Results: The SDS-PAGE pattern of LYS treated with papain under nonreducing conditions showed the presence of intact LYS that partially disappeared following reduction with \u03b2-mercaptoethanol, releasing IgE-reactive fragments as determined by Western blotting. MALDI-TOF\/TOF revealed that papain degraded LYS, giving rise to three IgE-binding fragments: LYS (22-129), LYS (34-96) and LYS (62-128) that likely remained linked through the disulfide bonds present in the LYS molecule. Conclusion: The combined administration of LYS with proteolytic enzymes such as papain may have developed a severe allergic reaction in the patient studied, underlining the importance of considering all the components and their interactions when drugs are to be consumed by allergic persons.<\/jats:p>","DOI":"10.1159\/000366101","type":"journal-article","created":{"date-parts":[[2014,10,29]],"date-time":"2014-10-29T07:34:00Z","timestamp":1414568040000},"page":"83-90","source":"Crossref","is-referenced-by-count":9,"title":["Anaphylaxis Induced by a Drug Containing Lysozyme and Papain: Influence of Papain on the IgE Response"],"prefix":"10.1159","volume":"165","author":[{"given":"Sara","family":"Bened\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inmaculada","family":"P\u00e9rez-Rangel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Lozano-Ojalvo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Molina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Dolores","family":"Iba\u00f1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosina","family":"L\u00f3pez-Fandi\u00f1o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iv\u00e1n","family":"L\u00f3pez-Exp\u00f3sito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,10,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idairyj.2006.06.002"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1997.tb00980.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1157\/13077741"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2008.10719707"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200800161"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)60843-5"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1157\/13101254"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2009.138"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12052"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1993.tb04754.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1021\/jf3001586"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201300442"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200435"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03722.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.022"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2000.00780.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038\/ni1558"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1159\/000334280"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159\/000024013"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.02.047"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.03.027"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041107"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/iaa\/article-pdf\/165\/2\/83\/3894347\/000366101.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/iaa\/article-pdf\/165\/2\/83\/3894347\/000366101.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:35:30Z","timestamp":1745429730000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000366101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":22,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,12,1]]},"published-print":{"date-parts":[[2014,12,18]]}},"URL":"https:\/\/doi.org\/10.1159\/000366101","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}