{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:17:10Z","timestamp":1759331830572,"version":"3.40.4"},"reference-count":19,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respiration"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; Biomarker-driven clinical trials in advanced non-small cell lung cancer (NSCLC) usually accept biopsy specimens only, as cytology specimens are supposed to be more challenging due to low neoplastic cell content and suboptimal DNA quantity. &lt;b&gt;&lt;i&gt;Objectives:&lt;\/i&gt;&lt;\/b&gt; We aimed to evaluate 2 aspects of bronchoscopic biopsy and cytology specimens: (1) the proportion of neoplastic cells and quantity of DNA extracted, and (2) the detection limit of the Scorpion amplification refractory mutation system on endoscopic samples obtained in daily clinical practice. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; We screened 679 patients with advanced-stage NSCLC for the presence of an activating EGFR mutation according to the guidelines of the European Society of Medical Oncology. Their diagnostic tumour tissue samples were characterized. A dilution experiment was performed to determine the minimal proportion of neoplastic cells for a reliable test result. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Surgical biopsies, bronchoscopic forceps biopsy samples and needle aspiration cytology specimens exhibited a median tumour cell proportion of 70 versus 30 versus 20% and a DNA quantity of 2,500 versus 1,610 versus 1,440 ng, respectively. The overall EGFR mutation rate was 11%, with no differences between different sample types. Dilution experiments showed that the detection limit depends on the type of mutation. A neoplastic cell content of at least 10 and 25% for exon 19 deletions and exon 21 L858R point mutation, respectively, was required for a true negative result. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; Bronchoscopic forceps biopsy and needle aspiration cytology specimens are suitable for accurate EGFR mutation analysis using single-gene quantitative real-time polymerase chain reaction. Technologies with a better analytical sensitivity are evolving and should consider these endoscopic tumour specimens.<\/jats:p>","DOI":"10.1159\/000366136","type":"journal-article","created":{"date-parts":[[2014,10,13]],"date-time":"2014-10-13T08:36:04Z","timestamp":1413189364000},"page":"371-377","source":"Crossref","is-referenced-by-count":6,"title":["Suitability of Small Bronchoscopic Tumour Specimens for Lung Cancer Genotyping"],"prefix":"10.1159","volume":"88","author":[{"given":"Christophe","family":"Dooms","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liesbet","family":"Vliegen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Vander Borght","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas","family":"Yserbyt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inge","family":"Hantson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Verbeken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Els","family":"Wauters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristiaan","family":"Nackaerts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Ninane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Vansteenkiste","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Vandenberghe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,10,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e31820517a3"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2011.10.017"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2013.03.001"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000357068"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mds121"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3181f1c8de"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019601"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-008-0665-y"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.45.3753"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdr489"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2696"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0561-CP"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.134"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03854.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3181ca12c4"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-2277"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017791"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2011-200275"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0000000000000104"}],"container-title":["Respiration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/366136","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/366136","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:35:47Z","timestamp":1745429747000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000366136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":19,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2014,11,1]]},"published-print":{"date-parts":[[2014,11,10]]}},"URL":"https:\/\/doi.org\/10.1159\/000366136","archive":["Portico"],"relation":{},"ISSN":["0025-7931","1423-0356"],"issn-type":[{"type":"print","value":"0025-7931"},{"type":"electronic","value":"1423-0356"}],"subject":[],"published":{"date-parts":[[2014]]}}}