{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T23:01:20Z","timestamp":1789599680149,"version":"build-2803163510"},"reference-count":22,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2014,11,19]],"date-time":"2014-11-19T00:00:00Z","timestamp":1416355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,11,19]],"date-time":"2014-11-19T00:00:00Z","timestamp":1416355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<b><i>Background:<\/i><\/b> Wnt5a is important for the development of various organs and postnatal cellular function. Little is known, however, about the role of <i>Wnt5a<\/i> in kidney development, although <i>WNT5A<\/i> mutations were identified in patients with Robinow syndrome, a genetic disease which includes developmental defects in kidneys. Our goal in this study was to determine the role of <i>Wnt5a<\/i> in kidney development. <b><i>Methods:<\/i><\/b> Whole-mount in situ hybridization was used to establish the expression pattern of <i>Wnt5a<\/i> during kidney development. Zebrafish with <i>wnt5a<\/i> knockdown and <i>Wnt5a<\/i> global knockout mice were used to identify kidney phenotypes. <b><i>Results:<\/i><\/b> In zebrafish, <i>wnt5a<\/i> knockdown resulted in glomerular cyst formation and dilated renal tubules. In mice, <i>Wnt5a<\/i> global knockout resulted in pleiotropic, but severe, kidney phenotypes, including agenesis, fused kidney, hydronephrosis and duplex kidney\/ureter. <b><i>Conclusions:<\/i><\/b> Our data demonstrated the important role of <i>Wnt5a<\/i> in kidney development. Disrupted Wnt5a resulted in kidney cysts in zebrafish and pleiotropic abnormal kidney development in mice.<\/jats:p>","DOI":"10.1159\/000368411","type":"journal-article","created":{"date-parts":[[2014,12,12]],"date-time":"2014-12-12T02:34:32Z","timestamp":1418351672000},"page":"80-88","source":"Crossref","is-referenced-by-count":29,"title":["Wnt5a Is Necessary for Normal Kidney Development in Zebrafish and Mice"],"prefix":"10.1159","volume":"128","author":[{"given":"Liwei","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Young","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quane","family":"Kan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria F.","family":"Chacon-Heszele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Kyoung","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"McKenna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rejji","family":"Kuruvilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua H.","family":"Lipschutz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2014,11,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.30785"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.1990.tb03534.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22156"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2653"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.01.003"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00394.2010"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.05.668"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn384"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2013.02.013"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012121236"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-6-45"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.30785"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.03.011"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2010010127"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01772"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22355"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21999"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/367380a0"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00491-14"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu397"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01604"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/368411","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/368411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T13:49:13Z","timestamp":1745416153000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000368411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,19]]},"references-count":22,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2014,12,1]]},"published-print":{"date-parts":[[2014,12,16]]}},"URL":"https:\/\/doi.org\/10.1159\/000368411","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5492854","asserted-by":"subject"}]},"ISSN":["1660-2129"],"issn-type":[{"value":"1660-2129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,19]]}}}