{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T12:54:59Z","timestamp":1754484899056,"version":"3.40.4"},"reference-count":18,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2015]]},"abstract":"<b><i>Objectives:<\/i><\/b> Little information is available concerning PCR-based direct detection of <i>Trichomonas<\/i> infections on archived Pap (Papanicolaou)-stained smears. This study investigates DNA extraction and amplification from archived Pap smears. <i>Trichomonas vaginalis<\/i> is a parasitic protozoan that infects the urogenital tract of women. <b><i>Study Design:<\/i><\/b> DNA from archived Pap-stained smears was successfully amplified using the nested PCR to investigate if it could be used for accurate detection and retrospective epidemiological investigations. <b><i>Results:<\/i><\/b> In our study, 98 (75.4%) out of 130 specimens of <i>T. vaginalis<\/i> Pap-stained smears were found to be positive by the nested PCR. Also, direct PCR on the archived Pap smears for identifying <i>T. vaginalis<\/i> gave a specificity of 100%. <b><i>Conclusion:<\/i><\/b> PCR-based Pap smears appear to offer an effective method to detect <i>Trichomonas<\/i> infection in archived samples, being rapid, highly specific and convenient for sampling, particularly in retrospective investigations.<\/jats:p>","DOI":"10.1159\/000369772","type":"journal-article","created":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T08:00:30Z","timestamp":1422259230000},"page":"104-108","source":"Crossref","is-referenced-by-count":6,"title":["Direct Diagnosis of <b><i>Trichomonas vaginalis<\/i><\/b> Infection on Archived Pap Smears Using Nested PCR"],"prefix":"10.1159","volume":"59","author":[{"given":"Hajar","family":"Ziaei Hezarjaribi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahbobeh","family":"Taghavi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdi","family":"Fakhar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirzad","family":"Gholami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2015,1,24]]},"reference":[{"doi-asserted-by":"publisher","key":"ref1","DOI":"10.3201\/eid0706.010603"},{"doi-asserted-by":"publisher","key":"ref2","DOI":"10.1097\/00007435-199503000-00003"},{"doi-asserted-by":"publisher","key":"ref3","DOI":"10.1086\/514728"},{"doi-asserted-by":"publisher","key":"ref4","DOI":"10.1016\/0002-9378(89)90851-X"},{"doi-asserted-by":"publisher","key":"ref5","DOI":"10.1001\/jama.1988.03720080057028"},{"doi-asserted-by":"publisher","key":"ref6","DOI":"10.1258\/095646204322764271"},{"doi-asserted-by":"publisher","key":"ref7","DOI":"10.1086\/342060"},{"doi-asserted-by":"publisher","key":"ref8","DOI":"10.1016\/S0001-706X(00)00180-7"},{"doi-asserted-by":"publisher","key":"ref9","DOI":"10.1128\/CMR.17.4.794-803.2004"},{"doi-asserted-by":"publisher","key":"ref10","DOI":"10.1126\/science.95.2469.438"},{"doi-asserted-by":"publisher","key":"ref11","DOI":"10.1097\/01.OLQ.0000079525.04451.05"},{"doi-asserted-by":"publisher","key":"ref12","DOI":"10.1002\/(SICI)1097-0339(199812)19:6<437::AID-DC6>3.0.CO;2-A"},{"doi-asserted-by":"publisher","key":"ref13","DOI":"10.1136\/jcp.46.4.378"},{"doi-asserted-by":"publisher","key":"ref14","DOI":"10.1177\/0956462414534833"},{"doi-asserted-by":"publisher","key":"ref15","DOI":"10.1159\/000333994"},{"doi-asserted-by":"publisher","key":"ref16","DOI":"10.1097\/00019606-199406000-00008"},{"doi-asserted-by":"publisher","key":"ref17","DOI":"10.1136\/jcp.2007.052688"},{"doi-asserted-by":"publisher","key":"ref18","DOI":"10.1002\/1098-0997(2000)8:5<248::AID-IDOG1028>3.0.CO;2-B"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/369772","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/369772","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:05:38Z","timestamp":1745431538000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000369772"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":18,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,2,1]]},"published-print":{"date-parts":[[2015,4,17]]}},"URL":"https:\/\/doi.org\/10.1159\/000369772","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"type":"print","value":"0001-5547"},{"type":"electronic","value":"1938-2650"}],"subject":[],"published":{"date-parts":[[2015]]}}}