{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:57:31Z","timestamp":1790035051853,"version":"4.0.1"},"reference-count":30,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Res Paediatr"],"published-print":{"date-parts":[[2015]]},"abstract":"<b><i>Background:<\/i><\/b> Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency leads to accumulation of steroid precursors and adrenal androgens. These steroids may have a biological effect on the steroid receptor with clinical consequences on diagnostics and treatment in CAH patients. Therefore, we analysed the effect of accumulated steroids [17-hydroxyprogesterone (17OHP), progesterone, androstenedione and testosterone] on aldosterone-mediated transactivation of the human mineralocorticoid receptor (hMR). <b><i>Methods:<\/i><\/b> A transactivation assay using transiently transfected COS7 cells was employed. Cells were co-transfected with hMR-cDNA, MMTV-luciferase and renilla-luciferase expression vectors. Transfected cells were incubated with six different steroid concentrations in addition to aldosterone (10<sup>-10<\/sup><smlcap>M<\/smlcap>). Luciferase and renilla activities were measured to quantify hMR transactivation. <b><i>Results:<\/i><\/b> Linear regression analysis showed statistically significant linear inhibition of transactivation of the hMR by 10<sup>-10<\/sup><smlcap>M<\/smlcap> aldosterone in the presence of increasing 17OHP [F(1,5) = 11.34, p = 0.019] and progesterone [F(1,5) = 11.08, p = 0.021] concentrations. In contrast, neither androstenedione nor testosterone affected hMR transactivation by aldosterone at a concentration of 10<sup>-10<\/sup><smlcap>M<\/smlcap>. <b><i>Conclusion:<\/i><\/b> Our study shows for the first time that neither androstenedione nor testosterone has a biological effect on aldosterone-mediated transactivation of the hMR. 17OHP and progesterone have an anti-mineralocorticoid effect in vitro that may clinically lead to an increased requirement of mineralocorticoids in poorly controlled CAH patients.<\/jats:p>","DOI":"10.1159\/000374112","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T21:02:11Z","timestamp":1429131731000},"page":"414-421","source":"Crossref","is-referenced-by-count":24,"title":["Influence of 17-Hydroxyprogesterone, Progesterone and Sex Steroids on Mineralocorticoid Receptor Transactivation in Congenital Adrenal Hyperplasia"],"prefix":"10.1159","volume":"83","author":[{"given":"Christiaan F.","family":"Mooij","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Parajes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karijn J.","family":"Pijnenburg-Kleizen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiebke","family":"Arlt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3402-4727","authenticated-orcid":false,"given":"Nils","family":"Krone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hedi L.","family":"Claahsen-van der Grinten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2015,4,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000327794"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.21.3.0398"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra021561"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-2631"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-0917"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1136\/adc.56.3.208"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(74)80340-9"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106052"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1460789"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-1161"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-15-10-1194"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1210\/endo-102-6-1686"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-62-5-934"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.0920560"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1982.tb00792.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(93)90072-H"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5476.119"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.2973"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00958.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1210\/mend.7.4.8388999"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1510397"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1621\/nrs.05012"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1210\/endo-125-1-192"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.3.1086"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1320305"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100374200"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2009.39"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024204"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2009.03690.x"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-0915"}],"container-title":["Hormone 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