{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T16:04:20Z","timestamp":1778947460744,"version":"3.51.4"},"reference-count":35,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>RIG-I-like receptors (RLRs) are cellular sensor proteins that detect certain RNA species produced during viral infections. RLRs activate a signaling cascade that results in the production of IFN-\u03b2 as well as several other cytokines with antiviral and proinflammatory activities. We explored the potential of different constructs based on RLRs to induce the IFN-\u03b2 pathway and create an antiviral state in type I IFN-unresponsive models. A chimeric construct composed of RIG-I 2CARD and the first 200 amino acids of MAVS (2CARD-MAVS200) showed an enhanced ability to induce IFN-\u03b2 when compared to other stimulatory constructs. Furthermore, this human chimeric construct showed a superior ability to activate IFN-\u03b2 expression in cells from various species. This construct was found to overcome the restrictions of blocking IFN-\u03b2 induction or signaling by a number of viral IFN-antagonist proteins. Additionally, the antiviral activity of this chimera was demonstrated in influenza virus and HBV infection mouse models using adeno-associated virus (AAV) vectors as a delivery vehicle. We propose that AAV vectors expressing 2CARD-MAVS200 chimeric protein can reconstitute IFN-\u03b2 induction and recover a partial antiviral state in different models that do not respond to recombinant IFN-\u03b2 treatment.<\/jats:p>","DOI":"10.1159\/000375262","type":"journal-article","created":{"date-parts":[[2015,5,4]],"date-time":"2015-05-04T21:01:23Z","timestamp":1430773283000},"page":"466-481","source":"Crossref","is-referenced-by-count":12,"title":["A RIG-I 2CARD-MAVS200 Chimeric Protein Reconstitutes IFN-\u03b2 Induction and Antiviral Response in Models Deficient in Type I IFN 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