{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:48:16Z","timestamp":1790146096543,"version":"4.0.1"},"reference-count":30,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sex Dev"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Spermatogonia contain processing bodies that harbor P-element-induced wimpy testis (Piwi) proteins. Piwi proteins are associated specifically with Piwi-interacting RNAs to silence transposable DNA elements. Loss-of-function mutations in the Piwi pathway lead to derepression of transposable elements, resulting in defective spermatogenesis. Furthermore, deletion of gametocyte-specific factor 1 &lt;i&gt;(GTSF1)&lt;\/i&gt;, a factor involved in Piwi-mediated transcriptional repression, causes male-specific sterility and derepression of LINE-1 (L1) retrotransposons. No previous studies have examined &lt;i&gt;GTSF1&lt;\/i&gt;, L1 and&lt;i&gt; PIWIL4&lt;\/i&gt; expression in cryptorchidism. We examined transposon-silencing genes and L1 transposon expression in testicular biopsies with Affymetrix microarrays and immunohistology. Seven members of the Tudor gene family, 3 members of the DEAD-box RNA helicase family, and the &lt;i&gt;GTSF1&lt;\/i&gt; gene were found to show significantly lower RNA signals in the high-infertility-risk group. In the immunohistochemical analysis, patients from the low-infertility-risk group showed coherently stronger staining for GTSF1 and PIWIL4 proteins and weaker staining for L1 transposon when compared to the high-infertility-risk samples. These new findings provide first evidence consistent with the idea that infertility in cryptorchidism is a consequence of alterations in the Piwi pathway and transposon derepression induced by the impaired function of mini-puberty.<\/jats:p>","DOI":"10.1159\/000375351","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T07:36:19Z","timestamp":1426491379000},"page":"98-104","source":"Crossref","is-referenced-by-count":37,"title":["Piwi-Pathway Alteration Induces LINE-1 Transposon Derepression and Infertility Development in Cryptorchidism"],"prefix":"10.1159","volume":"9","author":[{"given":"Faruk","family":"Hadziselimovic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils O.","family":"Hadziselimovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Demougin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunthild","family":"Krey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduard","family":"Oakeley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2015,3,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1142612"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297913060059"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2011.05.002"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911640106"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0338"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1391"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1101\/gad.221150.113"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1186\/1868-7083-4-23"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2008.05.063"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159\/000249147"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159\/000335188"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159\/000323955"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)06274-2"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-993194"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000181506.97839.b0"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-014-3558-y"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1126\/science.1089670"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.12.001"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1902110"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1640708"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baq030"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1140179"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.10.012"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041718"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1101\/gad.236414.113"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015447108"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401855101"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2009.0258"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth449"}],"container-title":["Sexual Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/375351","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/375351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:41:49Z","timestamp":1745430109000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000375351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":30,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,3,1]]},"published-print":{"date-parts":[[2015,3,19]]}},"URL":"https:\/\/doi.org\/10.1159\/000375351","archive":["Portico"],"relation":{},"ISSN":["1661-5425","1661-5433"],"issn-type":[{"value":"1661-5425","type":"print"},{"value":"1661-5433","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}