{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:08:41Z","timestamp":1762506521237,"version":"3.40.4"},"reference-count":28,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Obes Facts"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective: &lt;\/i&gt;&lt;\/b&gt;This study was initiated to evaluate the effects of Roux-en-Y gastric bypass surgery on renal gluconeogenesis in type 2 diabetic rats and its relationship with hormonal parameters. &lt;b&gt;&lt;i&gt;Methods&lt;\/i&gt;&lt;\/b&gt;: Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ; 35 mg\/kg) combined with a high-fat diet. They were then randomly divided into three groups: diabetes model group (DM group, n = 8), sham Roux-en-Y gastric bypass group (SRYGB group, n = 8), and Roux-en-Y gastric bypass group (RYGB group, n = 14). Another 8 normal rats comprised the normal control group (NC group, n = 8). Body weight, glucose, serum lipid, insulin, glucagon-like peptide-1 (GLP-1), leptin, and adiponectin were measured pre- and postoperatively. Glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), insulin receptor-\u03b1 (IR-\u03b1), insulin receptor-\u03b2 (IR-\u03b2), and glycogen synthase kinase 3 beta (Gsk3b) were measured in renal cortex by using RT-PCR and Western immune-blot analyses on the 4th week after operation. &lt;b&gt;&lt;i&gt;Results&lt;\/i&gt;&lt;\/b&gt;: Following RYGB surgery, surgery-treated rats showed significantly improved oral glucose tolerance, dyslipidemia and insulin resistance as well as increased post-gavage insulin levels and serum circulating levels of GLP-1 and adiponectin. RT-PCR and Western immune-blot analyses showed PEPCK and G6Pase protein and mRNA to be significantly decreased in the renal cortex in the RYGB group (p &lt; 0.05 vs. DM or SRYGB group); in addition, IR-\u03b1 and Gsk3b phosphorylation levels increased in the RYGB group (p &lt; 0.05 vs. DM or SRYGB group). &lt;b&gt;&lt;i&gt;Conclusion&lt;\/i&gt;&lt;\/b&gt;: Down-regulation of renal gluconeogenic enzymes might be a potential mechanism in hypoglycemia. An improved insulin signal pathway in the renal cortex and increased circulating adiponectin concentrations may contribute to the decline of renal gluconeogenesis following RYGB surgery.<\/jats:p>","DOI":"10.1159\/000381163","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T16:29:44Z","timestamp":1427387384000},"page":"110-124","source":"Crossref","is-referenced-by-count":10,"title":["Down-Regulation of Renal Gluconeogenesis in Type II Diabetic Rats Following Roux-en-Y Gastric Bypass Surgery: A Potential Mechanism in Hypoglycemic Effect"],"prefix":"10.1159","volume":"8","author":[{"given":"Yi","family":"Wen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Tao","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Feng","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Jun","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2015,3,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2009.10.001"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2011.10.029"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-008-1157-y"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1001\/jama.292.14.1724"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa066603"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-009-0169-1"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.051308.105148"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60401-2"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006121410"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2005.05.004"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181904af0"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159\/000351018"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200225"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200111"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00041.2004"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00104.2009"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00116.2001"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2008.12.031"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8227(11)70016-X"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2011.01511.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00763-08"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3109\/10799893.2012.660531"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.090266"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabet.2008.09.001"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.11.070"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1042\/BST0340798"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.26.12.3315"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI14120"}],"container-title":["Obesity Facts"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/381163","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/381163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:50:30Z","timestamp":1745430630000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000381163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":28,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000381163","archive":["Portico"],"relation":{},"ISSN":["1662-4025","1662-4033"],"issn-type":[{"type":"print","value":"1662-4025"},{"type":"electronic","value":"1662-4033"}],"subject":[],"published":{"date-parts":[[2015]]}}}