{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T04:18:21Z","timestamp":1777695501496,"version":"3.51.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T00:00:00Z","timestamp":1436227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T00:00:00Z","timestamp":1436227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fetal Diagn Ther"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Fetal amniotic membranes (FM) have been shown to preserve spinal cord histology in the fetal sheep model of myelomeningocele (MMC). This study compares the effectiveness of placenta-derived mesenchymal stromal cells (PMSCs) from early-gestation versus term-gestation placenta to augment FM repair to improve distal motor function in a sheep model. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Fetal lambs (n = 4) underwent surgical MMC creation followed by repair with FM patch with term-gestation PMSCs (n = 1), FM with early-gestation PMSCs (n = 1), FM only (n = 1), and skin closure only (n = 1). Histopathology and motor assessment was performed. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Histopathologic analysis demonstrated increased preservation of spinal cord architecture and large neurons in the lamb repaired with early-gestation cells compared to all others. Lambs repaired with skin closure only, FM alone, and term-gestation PMSCs exhibited extremely limited distal motor function; the lamb repaired with early-gestation PMSCs was capable of normal ambulation. &lt;b&gt;&lt;i&gt;Discussion:&lt;\/i&gt;&lt;\/b&gt; This pilot study is the first in vivo comparison of different gestational-age placenta-derived stromal cells for repair in the fetal sheep MMC model. The preservation of large neurons and markedly improved motor function in the lamb repaired with early-gestation cells suggest that early-gestation placental stromal cells may exhibit unique properties that augment in utero MMC repair to improve paralysis.<\/jats:p>","DOI":"10.1159\/000433427","type":"journal-article","created":{"date-parts":[[2015,7,6]],"date-time":"2015-07-06T21:02:24Z","timestamp":1436216544000},"page":"179-185","source":"Crossref","is-referenced-by-count":39,"title":["Age Does Matter: A Pilot Comparison of Placenta-Derived Stromal Cells for in utero Repair of Myelomeningocele Using a Lamb Model"],"prefix":"10.1159","volume":"39","author":[{"given":"Erin G.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin A.","family":"Keller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee","family":"Lankford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D.","family":"Pivetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinjiro","family":"Hirose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana L.","family":"Farmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2985-3627","authenticated-orcid":false,"given":"Aijun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2015,7,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1014379"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2013.09.043"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2012.21.6.290"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1080\/09273970802405493"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1586\/eop.10.63"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1424-6"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2007.00252.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.4103\/0975-5950.94469"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.08.033"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2012.03.024"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.01.101"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2013.06.019"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v4.i6.53"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.02.110"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(97)90603-5"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2015.01.002"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2006.23.36"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038\/nm0495-342"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2004.07.001"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14636"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1757"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00497-5"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2014.10.021"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.3727\/096368913X667709"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2014.10.007"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2000.108867"}],"container-title":["Fetal Diagnosis and Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/433427","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/433427","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:13:44Z","timestamp":1745432024000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000433427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,7]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000433427","archive":["Portico"],"relation":{},"ISSN":["1015-3837","1421-9964"],"issn-type":[{"value":"1015-3837","type":"print"},{"value":"1421-9964","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,7]]}}}