{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:03:31Z","timestamp":1791338611534,"version":"4.3.1"},"reference-count":32,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2015]]},"abstract":"We explored the regulatory mechanism of Leloir pathway genes in <i>Streptococcus pneumoniae<\/i> D39. Here, we demonstrate that the expression of <i>galKT<\/i> is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of <i>galKT<\/i>, as a transcriptional activator of <i>galKT<\/i> in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5\u2032-GATAGTTTAGTAAAATTTT-3\u2032) for the transcriptional regulator GalR in the promoter region of <i>galK<\/i>, which is also highly conserved in other streptococci. Growth comparison of D39 &#x0394;<i>galK<\/i> with the D39 wild type grown in the presence of galactose shows that <i>galK<\/i> is required for the proper growth of <i>S. pneumoniae<\/i> on galactose.<\/jats:p>","DOI":"10.1159\/000439429","type":"journal-article","created":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T22:01:19Z","timestamp":1446847279000},"page":"363-371","source":"Crossref","is-referenced-by-count":9,"title":["GalR Acts as a Transcriptional Activator of <b><i>galKT<\/i><\/b> in the Presence of Galactose in <b><i>Streptococcus pneumoniae<\/i><\/b>"],"prefix":"10.1159","volume":"25","author":[{"given":"Muhammad","family":"Afzal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulman","family":"Shafeeq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irfan","family":"Manzoor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar P.","family":"Kuipers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2015,11,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.6047-6052.2004"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3791\/52649"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.083899-0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01370-14"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00434-9"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-5-r37"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033320"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.08.008"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026707"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1994.tb00136.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20820"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1932"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2003.036921"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.24.8340-8349.2005"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1076"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2009.00564.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28521-0"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01148-06"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)01344-X"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.49.30860"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61204-6"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.gdata.2015.02.004"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.051359-0"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057586"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07758.x"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.01.009"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061217"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020628909429"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.01003.x"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01624-09"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01013-06"}],"container-title":["Microbial Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/439429","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/439429","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/439429","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:49:40Z","timestamp":1745430580000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000439429"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":32,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2016,2,1]]},"published-print":{"date-parts":[[2016,2,23]]}},"URL":"https:\/\/doi.org\/10.1159\/000439429","archive":["Portico"],"relation":{},"ISSN":["2673-1665","2673-1673"],"issn-type":[{"value":"2673-1665","type":"print"},{"value":"2673-1673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}