{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:51:48Z","timestamp":1787017908832,"version":"build-2736575974"},"reference-count":22,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2015,10,17]],"date-time":"2015-10-17T00:00:00Z","timestamp":1445040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2015,10,17]],"date-time":"2015-10-17T00:00:00Z","timestamp":1445040000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Urol Int"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; To evaluate the effect of tissue inhibitor of metalloproteinase-1 small interfering RNA (TIMP-1 siRNA) transfected fibroblasts (FB) for urethral reconstruction. &lt;b&gt;&lt;i&gt;Materials and Methods:&lt;\/i&gt;&lt;\/b&gt; A ventral urethral mucosal defect was created. Substitution urethroplasty was performed with small intestinal submucosa (SIS) alone (8 rabbits, group 1), autogenic oral keratinocytes (OK)-seeded SIS (8 rabbits, group 2) or autogenic OK and TIMP-1 siRNA transfected FB-seeded SIS (8 rabbits, group 3). At 1 and 6 months after surgery (4 rabbits at each time point), retrograde urethrogram and histologic analysis were performed to evaluate the outcomes of urethroplasty. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; TIMP-1 siRNA transfected FB decreased the secretion of type I collagen. Under retrograde urethrography, 5 rabbits in group 1, 6 in group 2 and 7 in group 3 maintained a wide urethral caliber. Histologically, inflammation and fibrosis were observed at 6 months in group 1. The speed of urothelium, smooth muscle and vessel regeneration in group 3 was faster than that in group 2. Comparison of smooth muscle-to-collagen ratio, epithelial layers, smooth muscle content and microvessel density among three groups revealed a significant increase (p &lt; 0.05). &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; TIMP-1 siRNA transfected FB could be used as a source of seed cell for urethral tissue engineering and could prevent the proliferation of urethral scar tissue.<\/jats:p>","DOI":"10.1159\/000440667","type":"journal-article","created":{"date-parts":[[2015,10,16]],"date-time":"2015-10-16T19:45:46Z","timestamp":1445024746000},"page":"223-230","source":"Crossref","is-referenced-by-count":15,"title":["Urethral Reconstruction with Small Intestinal Submucosa Seeded with Oral Keratinocytes and TIMP-1 siRNA Transfected Fibroblasts in a Rabbit Model"],"prefix":"10.1159","volume":"96","author":[{"given":"Hailin","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinglong","family":"Sa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minkai","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangguo","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2015,10,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.07230.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000360796"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000430500"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2005.04.045"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(98)00114-9"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.01.099"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2013.01.041"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2013.11.1102"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2003.378"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159\/000374028"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.396"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.375"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2011.09.001"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(01)00620-9"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.09.017"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/nbt936"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115083109"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2012.07.041"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2008.01.061"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2003.04860.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2006.05.007"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/561410"}],"container-title":["Urologia Internationalis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/440667","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/440667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T13:51:59Z","timestamp":1745416319000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000440667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,17]]},"references-count":22,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000440667","archive":["Portico"],"relation":{},"ISSN":["0042-1138","1423-0399"],"issn-type":[{"value":"0042-1138","type":"print"},{"value":"1423-0399","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,17]]}}}