{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:29:40Z","timestamp":1747193380532,"version":"3.40.5"},"reference-count":26,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; The Wnt\/\u03b2-catenin signaling pathway has been noted to be upregulated in head and neck cancers, including oropharyngeal squamous cell carcinoma (OSCC). This study compared the efficacy of \u03b2-catenin immunohistochemistry (IHC), p16 IHC and automated human papillomavirus (HPV) in situ hybridization (ISH) in OSCC. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Sixty-eight OSCCs (48 surgical specimens and 20 fine-needle aspirations) were evaluated. Nuclear staining only of \u03b2-catenin was assessed as 0-3+ intensity (relative to controls of benign squamous mucosa). p16 was interpreted as positive if 70% of tumor cells showed brown nuclear and cytoplasmic staining. HPV ISH was interpreted as positive if a minimum of one tumor cell showed brown punctate dot-like nuclear positivity. p16 IHC and HPV ISH were then correlated with \u03b2-catenin staining. HPV ISH was used as the gold standard. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Twenty-five of 48 surgical specimens (52.1%) and 11 of 20 cell blocks (55%) stained positively for \u03b2-catenin, making a total of 36 of 68 (52.9%) staining positively for \u03b2-catenin, as compared to 61.7% positive for p16 IHC and 70.6% positive by automated HPV ISH, the gold standard method for OSCC diagnosis. &amp;#x03C7;&lt;sup&gt;2&lt;\/sup&gt; analysis revealed no significant correlation between \u03b2-catenin and HPV ISH (p &gt; 0.05) and demonstrated a strong correlation between p16 and HPV ISH (p &lt; 0.05). &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; \u03b2-Catenin IHC is not a sensitive or specific marker of HPV and is unlikely to be a useful adjunct to p16 IHC or HPV ISH in the setting of advanced OSCC. However, as this study focused on samples of advanced OSCC, \u03b2-catenin IHC may still find some use in the diagnosis of early-stage OSCC.<\/jats:p>","DOI":"10.1159\/000443602","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T22:01:47Z","timestamp":1454709707000},"page":"479-484","source":"Crossref","is-referenced-by-count":6,"title":["\u03b2-Catenin Expression in Oropharyngeal Squamous Cell Carcinomas: Comparison and Correlation with p16 and Human Papillomavirus in situ Hybridization"],"prefix":"10.1159","volume":"59","author":[{"given":"Julum","family":"Nwanze","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3258-2511","authenticated-orcid":false,"given":"Alessandra C.","family":"Schmitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Momin T.","family":"Siddiqui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,2,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1994.1493"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199912)22:1<1021::AID-BIES6>3.0.CO;2-P"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23069"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/path.4383"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-009-0100-y"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-11-0287"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-09-0345"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.29039"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000353225"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.1.42"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601124"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/948264"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.01.016"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067361"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.52.633"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1186\/1746-1596-9-145"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.030502"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1097\/01.MP.0000085760.74313.DD"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2008.00710.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.1.58"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH720>3.0.CO;2-3"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i16.2398"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2014.1828"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990812)82:4<504::AID-IJC6>3.0.CO;2-6"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.171610498"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.51.12.891"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/443602","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/443602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:39:23Z","timestamp":1745429963000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000443602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000443602","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"type":"print","value":"0001-5547"},{"type":"electronic","value":"1938-2650"}],"subject":[],"published":{"date-parts":[[2015]]}}}