{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:08:05Z","timestamp":1790518085843,"version":"4.1.0"},"reference-count":35,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Cholera epidemics are caused by &lt;i&gt;Vibrio cholerae&lt;\/i&gt; serogroups O1 and O139, whereas strains collectively known as non-O1\/non-O139 &lt;i&gt;V. cholerae&lt;\/i&gt; are found in cases of extraintestinal infections and bacteremia. The mechanisms and factors influencing the occurrence of bacteremia and survival of &lt;i&gt;V. cholerae&lt;\/i&gt; in normal human serum have remained unclear. We found that naturally occurring IgG recognizing &lt;i&gt;V. cholerae&lt;\/i&gt; outer membrane protein U (OmpU) mediates a serum-killing effect in a complement C1q-dependent manner. Moreover, outer membrane vesicles (OMVs) containing OmpU caused enhanced survival of highly serum-sensitive classical &lt;i&gt;V. cholerae&lt;\/i&gt; in a dose-dependent manner. OMVs from wild-type and &lt;i&gt;ompU &lt;\/i&gt;mutant &lt;i&gt;V. cholerae&lt;\/i&gt; thereby provided a novel means to verify by extracellular transcomplementation the involvement of OmpU. Our data conclusively indicate that loss, or reduced expression, of OmpU imparts resistance to &lt;i&gt;V. cholerae&lt;\/i&gt; towards serum killing. We propose that the difference in OmpU protein levels is a plausible reason for differences in serum resistance and the ability to cause bacteremia observed among &lt;i&gt;V. cholerae&lt;\/i&gt; biotypes. Our findings provide a new perspective on how naturally occurring antibodies, perhaps induced by members of the microbiome, may play a role in the recognition of pathogens and the provocation of innate immune defense against bacteremia.<\/jats:p>","DOI":"10.1159\/000443646","type":"journal-article","created":{"date-parts":[[2016,3,2]],"date-time":"2016-03-02T22:01:23Z","timestamp":1456956083000},"page":"269-283","source":"Crossref","is-referenced-by-count":33,"title":["Naturally Occurring IgG Antibodies Provide Innate Protection against &lt;b&gt;&lt;i&gt;Vibrio cholerae &lt;\/i&gt;&lt;\/b&gt;Bacteremia by Recognition of the Outer Membrane Protein U"],"prefix":"10.1159","volume":"8","author":[{"given":"Kyaw Min","family":"Aung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika E.","family":"Sj\u00f6str\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"von 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