{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T10:17:30Z","timestamp":1779185850003,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Blood Purif"],"published-print":{"date-parts":[[2016]]},"abstract":"<b><i>Background:<\/i><\/b> We tested the effect of uremia on red blood cell (RBC) eryptosis, CD14<sup>++<\/sup>\/CD16<sup>+<\/sup> monocytes and erythrophagocytosis. <b><i>Design:<\/i><\/b> RBC and monocytes from chronic kidney disease (CKD) stages 3\/4 (P-CKD3\/4) or hemodialysis (HD) patients and healthy controls (HCs) cells incubated with sera pools from patients with CKD stages 2\/3 (S-CKD2\/3) or 4\/5 (S-CKD4\/5) were evaluated to assess eryptosis, monocyte phenotypes and reactive oxygen species (ROS) by cytometer. Erythrophagocytosis was evaluated by subsequent co-incubation of preincubated HC-monocytes and autologous-RBC. <b><i>Results:<\/i><\/b> HC-eryptosis (1.3 \u00b1 0.9%) was lower than in HD (4.3 \u00b1 0.5%) and HC-RBC incubated with S-CKD4\/5 (5.6 \u00b1 1%). CD14<sup>++<\/sup>\/CD16<sup>+<\/sup> were augmented in P-CKD3\/4 (34.6 \u00b1 8%) and HC-monocytes incubated with S-CKD4\/5 (26.4 \u00b1 7%) than in HC (5.4 \u00b1 1%). In these cells, ROS was increased (44.5 \u00b1 9%; control 9.6 \u00b1 2%) and inhibited by N-acetylcysteine (25 \u00b1 13%). Erythrophagocytosis was increased in CD14<sup>++<\/sup>\/CD16<sup>+<\/sup> (60.8 \u00b1 10%) than in CD14<sup>++<\/sup>\/CD16<sup>-<\/sup> (15.5 \u00b1 2%). <b><i>Conclusions:<\/i><\/b> Sera pools from CKD patients increase eryptosis and promote a proinflammatory monocyte phenotype. Both processes increased erythrophagocytosis, thereby suggesting a novel pathway for renal anemia.<\/jats:p>","DOI":"10.1159\/000443784","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T22:01:45Z","timestamp":1454709705000},"page":"317-323","source":"Crossref","is-referenced-by-count":40,"title":["Uremic Toxicity-Induced Eryptosis and Monocyte Modulation: The Erythrophagocytosis as a Novel Pathway to Renal Anemia"],"prefix":"10.1159","volume":"41","author":[{"given":"Natalia Borges","family":"Bonan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago M.","family":"Steiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoriya","family":"Kuntsevich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grazia Maria","family":"Virz\u00ec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Azevedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lia Sumie","family":"Nakao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fellype Carvalho","family":"Barreto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6697-4065","authenticated-orcid":false,"given":"Claudio","family":"Ronco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Thijssen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Kotanko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Pecoits-Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a N.","family":"Moreno-Amaral","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,2,6]]},"container-title":["Blood Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/443784","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/443784","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:41:09Z","timestamp":1745430069000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000443784"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000443784","archive":["Portico"],"relation":{},"ISSN":["0253-5068","1421-9735"],"issn-type":[{"value":"0253-5068","type":"print"},{"value":"1421-9735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}