{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T12:47:28Z","timestamp":1767703648942,"version":"3.40.4"},"reference-count":17,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2016]]},"abstract":"<b><i>Background:<\/i><\/b> Metallo-\u03b2-lactamases (MBLs) producing strains of <i>Acinetobacter baumannii<\/i> are serious etiological agents of hospital infections worldwide. Among the \u03b2- lactams, carbapenems are the most effective antibiotics used against <i>A. baumannii. <\/i>However, resistance to these drugs among clinical strains of <i>A. baumannii<\/i> has been increasing in recent years. In this study, the antimicrobial sensitivity patterns of <i>A. baumannii<\/i> strains isolated from eleven different hospitals in Tehran, Iran, and the prevalence of MBL genes (<i>bla-<\/i><sub><i>VIM<\/i><\/sub> and <i>bla-<\/i><sub><i>IMP<\/i><\/sub>) were determined. <b><i>Method:<\/i><\/b> During a period of 5 months, 176 isolates of <i>A. baumannii<\/i> were collected from different clinical specimens from hospitalized patients in Tehran. All isolates were confirmed by biochemical methods. The isolates were tested for antibiotic sensitivity by the Kirby-Bauer disk diffusion method. Following minimum inhibitory concentration determination, imipenem-resistant isolates were further tested for MBL production by the double disk synergy test (DDST) method. PCR assays were performed for the detection of the MBL genes <i>bla-<\/i><sub><i>IMP<\/i><\/sub> and <i>bla-<\/i><sub><i>VIM<\/i><\/sub>. <b><i>Results:<\/i><\/b> The DDST phenotypic method indicated that among the 169 imipenem-resistant isolates, 165 strains were MBL positive. The PCR assays revealed that 63 of the overall isolates (36%) carried the <i>bla-<\/i><sub><i>VIM<\/i><\/sub> gene and 70 strains (40%) harbored <i>bla-<\/i><sub><i>IMP<\/i><\/sub>. <b><i>Conclusions:<\/i><\/b> It is obvious that nosocomial infections associated with multidrug-resistant <i>Acinetobacter <\/i>spp. are on the rise. Therefore, the determination of antibiotic sensitivity patterns and screening for MBL production among <i>A. baumannii<\/i> isolates is important for controlling clinical <i>Acinetobacter<\/i> infections.<\/jats:p>","DOI":"10.1159\/000443825","type":"journal-article","created":{"date-parts":[[2016,4,9]],"date-time":"2016-04-09T02:55:52Z","timestamp":1460170552000},"page":"275-280","source":"Crossref","is-referenced-by-count":13,"title":["Antibiotic Resistance Patterns and a Survey of Metallo-\u03b2-Lactamase Genes Including <b><i>bla-<\/i><\/b><sub><i>IMP<\/i><\/sub> and <b><i>bla-<\/i><\/b><sub><i>VIM<\/i><\/sub> Types in <b><i>Acinetobacter baumannii<\/i><\/b> Isolated from Hospital Patients in Tehran"],"prefix":"10.1159","volume":"61","author":[{"given":"Samira","family":"Aghamiri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nour","family":"Amirmozafari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jalil","family":"Fallah Mehrabadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Babak","family":"Fouladtan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojtaba","family":"Hanafi Abdar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,4,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000351098"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01876-07"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1789"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1086\/650379"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3542-3547.2003"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.5812\/pedinfect.15439"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.00942.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000171920.44809.f0"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3129-3135.2005"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn454"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg276"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02030.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.4103\/0377-4929.64333"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.2924"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3205\/dgkh000216"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.002105-0"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/443825","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/443825","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:41:39Z","timestamp":1745430099000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000443825"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":17,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2016,8,22]]}},"URL":"https:\/\/doi.org\/10.1159\/000443825","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"type":"print","value":"0009-3157"},{"type":"electronic","value":"1421-9794"}],"subject":[],"published":{"date-parts":[[2016]]}}}