{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:32:38Z","timestamp":1761863558870,"version":"3.40.4"},"reference-count":27,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2016]]},"abstract":"<b><i>Background:<\/i><\/b> Stings from the Asian needle ant are an important cause of anaphylaxis in East Asia. A 23-kDa protein homologous to antigen 5 is the major allergen produced by these ants. In this study, we aimed to produce a recombinant antigen 5 allergen, Pac c 3. <b><i>Methods:<\/i><\/b> Recombinant Pac c 3 allergen from the Asian needle ant was expressed in <i>Pichia pastoris<\/i> and purified by ammonium sulfate precipitation and Ni affinity chromatography. IgE reactivity was demonstrated by ELISA and immunoblotting. <b><i>Results:<\/i><\/b> The recombinant protein was recognized in 5 of 6 (83.3%) serum samples from patients with demonstrated anaphylaxis to ants. IgE reactivity to an antigen 5 allergen from Asian needle ant venom sac extract was specifically inhibited by the recombinant protein. It was also able to inhibit IgE binding to the vespid allergen Ves v 5 by ImmunoCAP analysis, indicating the presence of cross-reactivity. <b><i>Conclusion:<\/i><\/b> A recombinant Pac c 3, cross-reactive with Ves v 5, from the Asian needle ant was successfully produced in the methylotrophic yeast <i>P. pastoris<\/i>. This protein could be useful for the development of component-resolved diagnostics.<\/jats:p>","DOI":"10.1159\/000444364","type":"journal-article","created":{"date-parts":[[2016,4,4]],"date-time":"2016-04-04T21:48:16Z","timestamp":1459806496000},"page":"93-100","source":"Crossref","is-referenced-by-count":19,"title":["IgE Reactivity of Recombinant Pac c 3 from the Asian Needle Ant <b><i>(Pachycondyla chinensis)<\/i><\/b>"],"prefix":"10.1159","volume":"169","author":[{"given":"Kyoung Yong","family":"Jeong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung-hee","family":"Yi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Son","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongpyo","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Hyun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai-Soon","family":"Yong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung-Won","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,4,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.1992.33.3.281"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.5021\/ad.2004.16.2.87"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70302-7"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.114341"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.124890"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.03181.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1021\/bi00536a034"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/prot.1160"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.48.060402.102812"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(77)90169-5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197807272990401"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.08.037"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020842"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02781.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2012.02847.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1385\/CRIAI:30:2:109"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.2174\/138920306775474112"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/arch.940220302"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.00085.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-015-0527-z"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1999.1082"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1208"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1021\/pr4008927"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2009.01.017"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2013.5.3.129"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.5656\/KSAE.2013.02.1.072"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1007\/BF01417460"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/444364","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/444364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:46:57Z","timestamp":1745430417000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000444364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":27,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,4,1]]},"published-print":{"date-parts":[[2016,5,3]]}},"URL":"https:\/\/doi.org\/10.1159\/000444364","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2016]]}}}