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Karger AG","issue":"2","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>We aimed to investigate the role of CCAAT enhancer-binding protein \u03b1 (C\/EBP\u03b1) in the pathogenesis of chronic myeloid leukemia (CML) and the mechanism underlying its effect. Bone marrow specimens from 50 patients with CML and peripheral blood specimens from 20 healthy individuals were collected. K562 cells were treated with imatinib. Subsequently, a stable cell line, K562-C\/EBP\u03b1, was constructed. Cell proliferation was assayed with cell counting kit-8, and mRNA levels of C\/EBP\u03b1, forkhead transcription factor FKHRL1 (Foxo3a) and Bim were detected by semiquantitative PCR. The correlation of C\/EBP\u03b1 and BCR-ABL was assessed by Spearman's correlation analysis. The results showed that C\/EBP\u03b1 mRNA levels were significantly reduced in CML patients compared with healthy subjects (p &lt; 0.001) and were negatively correlated with BCR-ABL1 (r = -0.5046, p &lt; 0.01). Additionally, imatinib enhanced the expression of C\/EBP\u03b1 in K562 cells compared with untreated cells (p &lt; 0.05). Overexpression of C\/EBP\u03b1 significantly decreased cell proliferation and upregulated the expressions of the apoptosis-related genes &lt;i&gt;Foxo3a&lt;\/i&gt; (p &lt; 0.01) and &lt;i&gt;Bim&lt;\/i&gt; (p &lt; 0.05) in K562 cells. In conclusion, C\/EBP\u03b1 expression was decreased in patients with CML. Imatinib enhances the expression of C\/EBP\u03b1 in K562 cells, and the overexpression of C\/EBP\u03b1 inhibits cell proliferation and increases apoptosis via the Foxo3a-Bim pathway.<\/jats:p>","DOI":"10.1159\/000444517","type":"journal-article","created":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T21:05:33Z","timestamp":1462827933000},"page":"65-70","source":"Crossref","is-referenced-by-count":4,"title":["Overexpression of CCAAT Enhancer-Binding Protein \u03b1 Inhibits the Growth of K562 Cells via the Foxo3a-Bim 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