{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T14:16:20Z","timestamp":1788617780712,"version":"build-2803163510"},"reference-count":31,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL) may include the lymphoid blast crisis of chronic myeloid leukemia (CML-BC). We applied fluorescence in situ hybridization (FISH) of the &lt;i&gt;BCR&lt;\/i&gt;-&lt;i&gt;ABL&lt;\/i&gt; fusion gene to peripheral blood and\/or bone marrow smears to determine whether the fusion was restricted to mononuclear cell nuclei or if segmented cell nuclei representing mature neutrophils also carried the fusion (Seg-FISH). Among 20 patients with Ph+ ALL without a prior diagnosis of CML, 9 were Seg-FISH+ and 11 were Seg-FISH-. Seg-FISH+ cases were characterized by a higher rate of p210-type &lt;i&gt;BCR&lt;\/i&gt;-&lt;i&gt;ABL&lt;\/i&gt; transcripts, higher white cell and blast counts, and a higher rate of myeloid and T-lymphoid antigen expression than Seg-FISH- cases, in addition to \u2018major route' cytogenetic abnormalities associated with CML-BC. Eighteen patients were treated with tyrosine kinase inhibitors (TKIs) either alone or in combination with multiagent chemotherapy, and 7 underwent allogeneic hematopoietic stem cell transplantation. Progression-free and overall survivals were greater in the Seg-FISH+ group than in the Seg-FISH- group. These results suggest that the Seg-FISH+ group represents lymphoid CML-BC that occurs de novo, while the Seg-FISH- represents Ph+ ALL in the strict sense, and the two groups are associated with survival when treated with TKIs or TKI-combined therapy.<\/jats:p>","DOI":"10.1159\/000445972","type":"journal-article","created":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T21:01:40Z","timestamp":1471554100000},"page":"157-166","source":"Crossref","is-referenced-by-count":20,"title":["Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia&lt;b&gt; &lt;\/b&gt;Is Separated into Two Subgroups Associated with Survival by &lt;b&gt;&lt;i&gt;BCR&lt;\/i&gt;&lt;\/b&gt;-&lt;b&gt;&lt;i&gt;ABL&lt;\/i&gt;&lt;\/b&gt; Fluorescence in situ Hybridization of Segmented Cell Nuclei: Report from a Single Institution"],"prefix":"10.1159","volume":"136","author":[{"given":"Yoshimasa","family":"Kamoda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyotaka","family":"Izumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Futoshi","family":"Iioka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Akasaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihiko","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiyuki","family":"Kishimori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyo","family":"Tsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiro","family":"Fukutsuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Okumura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiko","family":"Hayashida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2016,8,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199907153410306"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-051912"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05611.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0704"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.5.1536"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.03.2177"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-12-261586"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-08-2958"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-03-011908"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.28.1287"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.14694\/EdBook_AM.2015.35.e352"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.28433"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12079"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.4137\/CIN.S9258"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1159\/000084448"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.11406\/rinketsu.50.481"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25978"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.12936\/tenrikiyo.18-007"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.3109\/10428199309047856"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.26.9456"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.383"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21291"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11891"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403714"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-02-204693"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-07-234666"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-501569"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(15)00207-7"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2007.070050"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-012-1139-1"}],"container-title":["Acta 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