{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T16:22:09Z","timestamp":1792167729959,"version":"4.4.0"},"reference-count":14,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfus Med Hemother"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>&lt;b&gt;Background:&lt;\/b&gt; The performance of the multiplex Procleix Ultrio Elite assay as individual donor nucleic acid test (ID-NAT) for the detection of HIV-1, HIV-2, HCV, and HBV was evaluated in a retrospective, single center study. &lt;b&gt;Methods:&lt;\/b&gt; ID-NAT results of 21,181 blood donors, 984 tissue donors, 293 hematopoietic stem cell donors and 4 organ donors were reviewed in synopsis with results of serological screening and additional discriminatory and repetitive NAT in case of positive donors. &lt;b&gt;Results:&lt;\/b&gt; Specificity of the initial Procleix Ultrio Elite assay was 99.98% and after discriminatory testing 100.00%. Initially invalid results were observed in 75 of 21,181 blood donors (0.35%) but 16 of 984 tissue donors (1.62%, p &lt; 0.001) which included non-heart-beating (\u2018cadaveric') donors. All these had valid negative ID-NAT results after repeated testing or testing of 1:5 diluted specimens in case of tissue donors. Occult hepatitis B (defined here as HBV DNAemia without HBsAg detection) was demonstrated by ID-NAT in two anti-HBc-positive tissue donors and suspected in two other tissue donors, where a definite diagnosis was not achieved due to the insufficient sample volumes available. &lt;b&gt;Conclusion:&lt;\/b&gt; The Procleix Ultrio Elite assay proved to be specific, robust and rapid. Therefore, routine ID-NAT may also be feasible for organ and granulocyte donors.<\/jats:p>","DOI":"10.1159\/000446217","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T21:01:29Z","timestamp":1462222889000},"page":"177-182","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of the Procleix Ultrio Elite Assay and the Panther-System for Individual NAT Screening of Blood, Hematopoietic Stem Cell, Tissue and Organ Donors"],"prefix":"10.1159","volume":"43","author":[{"given":"Albert","family":"Heim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2016,5,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02204-08"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02203.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000357103"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03281.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s00103-013-1673-x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02732.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02804.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00197.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13193"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2012.01624.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2009.06.007"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12653"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.5812\/hepatmon.15275"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00166.x"}],"container-title":["Transfusion Medicine and Hemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/446217","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/446217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:05:28Z","timestamp":1745431528000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000446217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":14,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,5,31]]}},"URL":"https:\/\/doi.org\/10.1159\/000446217","archive":["Portico"],"relation":{},"ISSN":["1660-3796","1660-3818"],"issn-type":[{"value":"1660-3796","type":"print"},{"value":"1660-3818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}