{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:51:52Z","timestamp":1787017912922,"version":"build-2736575974"},"reference-count":17,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfus Med Hemother"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>&lt;b&gt;Background:&lt;\/b&gt; Blood product safety was significantly improved by the introduction of NAT testing in the late 1990s, resulting in a strong decrease of transfusion-transmitted infections (TTIs). Due to the occurrence of HIV-1 NAT test failures as a consequence of mismatch mutations in the amplicon regions of mono-target NAT assays, the Paul Ehrlich Institute mandated the implementation of multi-target NAT assays for HIV-1 in 2014. Commercial suppliers mostly developed dual-target NAT assays, with only one implementing a triple-target NAT assay. &lt;b&gt;Methods:&lt;\/b&gt; The HIV-1 triple-target NAT assay v3 (GFE Blut) was tested on mutated specimens and synthetic DNA bearing mutations that resulted in sample underquantification or false-negative test results. In addition, data from 2 years routine testing at three German Red Cross Blood centres were analysed. &lt;b&gt;Results:&lt;\/b&gt; The HIV-1 triple-target PCR could compensate for all mutations tested and could compensate the loss of one amplicon without a significant loss of sensitivity. Data from 2 years routine testing showed a solid performance. &lt;b&gt;Conclusion:&lt;\/b&gt; The HIV-1 triple-target v3 assay (GFE Blut) can compensate mutations in target sequences better than a dual-target assay and is applicable to high-throughput screening, thus increasing blood product safety.<\/jats:p>","DOI":"10.1159\/000446290","type":"journal-article","created":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T17:38:49Z","timestamp":1464025129000},"page":"183-189","source":"Crossref","is-referenced-by-count":4,"title":["Implementation of an HIV-1 Triple-Target NAT Assay in the Routine Screening at Three German Red Cross Blood Centres"],"prefix":"10.1159","volume":"43","author":[{"given":"Silke","family":"De Zolt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Thermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"Bangsow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Pichl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Jork","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijke","family":"Weber-Schehl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Hedges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingo","family":"Schupp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Unverzagt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"de Rue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Kurt","family":"Roth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2016,5,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890430114"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)06318-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2011.01506.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.2002.00383.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01718.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00103-012-1516-1"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02212.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03281.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000357103"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12298"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02203.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl255"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02477.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02942.x"}],"container-title":["Transfusion Medicine and Hemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/446290","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/446290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T14:05:55Z","timestamp":1745417155000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000446290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":17,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,5,31]]}},"URL":"https:\/\/doi.org\/10.1159\/000446290","archive":["Portico"],"relation":{},"ISSN":["1660-3796","1660-3818"],"issn-type":[{"value":"1660-3796","type":"print"},{"value":"1660-3818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}