{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:27:13Z","timestamp":1789914433017,"version":"4.0.1"},"reference-count":30,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background\/Aims:&lt;\/i&gt;&lt;\/b&gt; Lupus nephritis (LN) is a frequent complication and a major predictor of poor prognosis of systemic lupus erythematosus. Immune complex deposition and T cell infiltration are crucial events in LN pathogenesis. B7-1 (CD80), normally expressed by antigen-presenting cells, is one of the major co-stimulators of T-cell activation through the binding with its counter-receptors CD28 and cytotoxic T-lymphocyte antigen-4. Unexpectedly, B7-1 induction was described at the podocyte level in patients affected by different renal diseases, including LN. These observations suggested a novel exciting function for B7-1 as a biomarker of podocyte injury, and hence that B7-1 inhibitory drugs could serve as podocyte-targeted treatment of intractable renal diseases. However, subsequent studies hardly questioned the reliability of B7-1 detection assays and the therapeutic efficacy of B7-1 blockade in proteinuric patients, casting doubts on B7-1 expression by podocytes. Here, we thoroughly investigated whether B7-1 was indeed expressed by podocytes in LN, before even considering employing B7-1 blockade in patients with severe manifestations of LN and unfavourable prognosis. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Applying different immunohistochemical assays with 4 primary antibodies, we analysed kidney biopsies from 42 LN patients at different stages of the disease, and from NZB\/NZW mice, an LN model. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; B7-1 was not induced in podocytes in human and murine LN; instead its expression was confined to infiltrating inflammatory cells. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; B7-1 is not expressed by podocytes in LN. A renoprotective effect of B7-1 blockade in LN patients cannot be ruled out but, if confirmed, cannot be the result of an effect on podocyte B7-1.<\/jats:p>","DOI":"10.1159\/000446324","type":"journal-article","created":{"date-parts":[[2016,5,22]],"date-time":"2016-05-22T04:35:07Z","timestamp":1463891707000},"page":"129-138","source":"Crossref","is-referenced-by-count":11,"title":["Another Piece of the Puzzle of Podocyte B7-1 Expression: Lupus Nephritis"],"prefix":"10.1159","volume":"133","author":[{"given":"Rubina","family":"Novelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Gagliardini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Ruggiero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariela","family":"Benigni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Remuzzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2016,5,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4227"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/849684"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000108969.21691.5D"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1191\/0961203305lu2056oa"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1998.tb01242.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.2174\/138161206775193046"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012020133"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(03)00192-6"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20402"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2007080836"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-014-2957-6"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1304572"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013050518"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2015030266"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1400502"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2014.07.023"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111\/tri.12574"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2015091002"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1400502"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60342-9"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0000000000000137"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2015080947"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.11.6046"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3953"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1002\/art.30176"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1002\/art.27601"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1002\/art.38260"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1002\/art.38790"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2012.04.010"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102728"}],"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/446324","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/446324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:06:09Z","timestamp":1745431569000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000446324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":30,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,6,30]]}},"URL":"https:\/\/doi.org\/10.1159\/000446324","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.3996216","asserted-by":"subject"}]},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}