{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T13:22:09Z","timestamp":1778678529957,"version":"3.51.4"},"reference-count":42,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Minimally modified low-density lipoprotein (mmLDL) is a well-known risk factor for cardiovascular diseases. The present study was designed to investigate the role of mmLDL in the endothelium-dependent relaxation of mouse mesenteric arteries. A sensitive myograph system was employed to examine the endothelial function of mesenteric arteries. mRNA and protein expression levels were determined using real-time PCR and Western blotting, respectively. The ultramicrostructure of mesenteric vascular beds was investigated using a transmission electron microscope. The results showed that mmLDL significantly impaired the acetylcholine-induced (3 \u00d7 10\u2212&lt;sup&gt;10&lt;\/sup&gt; to 1 \u00d7 10\u2212&lt;sup&gt;4&lt;\/sup&gt;&lt;smlcap&gt;M&lt;\/smlcap&gt;) endothelium-dependent relaxation of mouse mesenteric arteries with markedly reduced pIC&lt;sub&gt;50&lt;\/sub&gt; (p &lt; 0.05) and R&lt;sub&gt;max&lt;\/sub&gt; values (p &lt; 0.001). In addition, mmLDL increased the levels of superoxide production and nitrotyrosine concentration and impaired the endothelial microstructure with decreased K&lt;sub&gt;Ca&lt;\/sub&gt;3.1 and K&lt;sub&gt;Ca&lt;\/sub&gt;2.3 expression. In conclusion, mmLDL increases superoxide and nitrotyrosine levels, damages endothelial microstructure with decreased K&lt;sub&gt;Ca&lt;\/sub&gt;3.1 and K&lt;sub&gt;Ca&lt;\/sub&gt;2.3 expression, and ultimately attenuates relaxation mediated by nitric oxide- and endothelium-derived hyperpolarizing factor.<\/jats:p>","DOI":"10.1159\/000447011","type":"journal-article","created":{"date-parts":[[2016,8,31]],"date-time":"2016-08-31T21:02:57Z","timestamp":1472677377000},"page":"58-71","source":"Crossref","is-referenced-by-count":7,"title":["Minimally Modified LDL-Induced Impairment of Endothelium-Dependent Relaxation in Small Mesenteric Arteries of 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