{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T01:39:04Z","timestamp":1767922744455,"version":"3.49.0"},"reference-count":24,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt;&lt;i&gt;Dermatophagoides pteronyssinus&lt;\/i&gt; is one of the most important perennial allergen sources worldwide. Molecular diagnostics using the commercially available major allergens (Der p 1 and Der p 2) in combination with Der p 10 do not detect house dust mite (HDM) sensitization in a number of cases when used alone. The objective was to evaluate the IgE reactivity profiles of these patients using an experimental immunoassay biochip. &lt;b&gt;&lt;i&gt;Methods: &lt;\/i&gt;&lt;\/b&gt;Sera of HDM-allergic patients (positive skin prick test, CAP class \u22651 for allergen extract, and positive intranasal provocation) were tested for IgE antibodies against Der p 1, Der p 2, and Der p 10 by ImmunoCAP fluorescence enzyme immunoassay. Negatively tested sera were examined by an experimental chip containing 13 microarrayed HDM allergens. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Of 97 patients tested, 16 showed negative results to Der p 1, Der p 2, and Der p 10. MeDALL chip evaluation revealed 5 patients monosensitized to Der p 23, and 11 patients were negative for all HDM MeDALL chip components. Seven sera were available for further testing, and 3 of them showed IgE reactivity to dot-blotted nDer p 1, and 2 reacted with high-molecular weight components (&gt;100 kDa) in nitrocellulose-blotted HDM extract when tested with &lt;sup&gt;125&lt;\/sup&gt;I-labeled anti-IgE in a RAST-based assay. The HDM extract-specific IgE levels of the 11 patients were &lt;3.9 kU\/l. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; Recombinant allergen-based IgE serology is of great value when conventional IgE diagnostics fails. Der p 23 is an important HDM allergen, especially when major allergens are negative. Therefore, it would be desirable to have Der p 23 commercially available. Further research concerning the prevalence and clinical significance of different HDM allergens is needed.<\/jats:p>","DOI":"10.1159\/000447694","type":"journal-article","created":{"date-parts":[[2016,8,9]],"date-time":"2016-08-09T21:01:41Z","timestamp":1470776501000},"page":"132-137","source":"Crossref","is-referenced-by-count":46,"title":["Real-Life Study for the Diagnosis of House Dust Mite Allergy - The Value of Recombinant Allergen-Based IgE Serology"],"prefix":"10.1159","volume":"170","author":[{"given":"Sven","family":"Becker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Schlederer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias F.","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mareike","family":"Haack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Vrtala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Resch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Lupinek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"Valenta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moritz","family":"Gr\u00f6ger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,8,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70271-4"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.1930.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01540.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-013-2516-y"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2008.02048.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-011-1826-1"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD001936.pub2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000104"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.09.004"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1994.tb02741.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202288"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.10.008"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-38411"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2012.04035.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1159\/000065179"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2745.2002.01455.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1159\/000442176"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2007.02786.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159\/000234724"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.09.031"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1159\/000066770"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1159\/000096114"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1159\/000324447"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1159\/000337654"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/447694","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/447694","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/447694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:55:30Z","timestamp":1745427330000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000447694"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,8,25]]}},"URL":"https:\/\/doi.org\/10.1159\/000447694","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}