{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:01:10Z","timestamp":1758632470405,"version":"3.40.5"},"reference-count":39,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>In 2009, a novel pandemic swine-origin influenza A (H1N1) virus caused a public emergency of international concern. Vaccination is the primary strategy for the control of influenza epidemics. However, the poor immunopotency of many vaccine antigens is a major barrier to the development of effective vaccines against influenza. Flagellin, a Toll-like receptor 5 (TLR5) ligand, has been used as an adjuvant to enhance the immunopotency of vaccines in preclinical studies. Here, we developed a recombinant candidate vaccine, HA1-2-fljB, in which the globular head of the hemagglutinin (HA) antigen (residues 62-284) from H1N1 virus was fused genetically to the N-terminus of &lt;i&gt;Salmonella typhimurium&lt;\/i&gt; &amp;#xFB02;jB. The recombinant HA1-2-fljB protein was expressed efficiently in&lt;i&gt; Escherichia coli&lt;\/i&gt;, and the immunogenicity and protective efficacy of recombinant HA1-2-fljB were evaluated in a mouse model. Immunization with HA1-2-fljB elicited robust IgG antibodies and neutralizing antibodies and completely protected the mice against infection by swine-origin influenza A\/swine\/Jangsu\/38\/2010 (H1N1). These results suggest that HA antigen placed at the N-terminus of flagellin is also an excellent starting point for creating a fusion HA1-2-fljB protein as a candidate vaccine, and the recombinant HA1-2-fljB protein will contribute to the development of a more effective vaccine against swine-origin influenza virus infection.<\/jats:p>","DOI":"10.1159\/000448895","type":"journal-article","created":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T21:01:38Z","timestamp":1475874098000},"page":"422-432","source":"Crossref","is-referenced-by-count":2,"title":["HA1-2-fljB Vaccine Induces Immune Responses against Pandemic Swine-Origin H1N1 Influenza Virus in Mice"],"prefix":"10.1159","volume":"26","author":[{"given":"Xilong","family":"Kang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongqin","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2685-6240","authenticated-orcid":false,"given":"Dan","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiming","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinan","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,10,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1530"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/90609"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.12.059"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.07.107"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425403"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0903810"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2009.0107"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00071-9"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/35074106"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.3.613"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.04.072"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1208"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.08.013"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/nature05495"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002\/biot.200900267"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100026"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1002842"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.01.005"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201300116"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.09.013"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020928"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1086\/517613"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.7.3914"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002156"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1086\/507544"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00251-13"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-013-2399-z"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.531"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002257"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1186\/s12896-015-0195-z"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.07.060"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.88.226"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.08.002"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.05.041"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1007\/82_2012_266"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00054-09"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041501"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-008-0253-0"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.09.088"}],"container-title":["Microbial 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