{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T22:36:04Z","timestamp":1779316564844,"version":"3.51.4"},"reference-count":29,"publisher":"S. Karger AG","issue":"3-4","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2016]]},"abstract":"<b><i>Objectives:<\/i><\/b> Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y<sub>2<\/sub> nucleotide receptor (P2Y<sub>2<\/sub>R) in neointimal hyperplasia. <b><i>Approach and Results:<\/i><\/b> Vascular injury was induced by the implantation of a polyethylene cuff around the femoral artery in wild-type and P2Y<sub>2<\/sub>R-deficient mice (P2Y<sub>2<\/sub>R<sup>-\/-<\/sup>). Electron microscopy was used to analyze monocyte and lymphocyte influx to the intima 36 h after injury. Compared to wild-type littermates, P2Y<sub>2<\/sub>R<sup>-\/-<\/sup> mice exhibited a 3-fold decreased number of mononuclear leukocytes invading the intima (p < 0.05). Concomitantly, the migration of smooth muscle cells was decreased by more than 60% (p < 0.05), resulting in a sharp inhibition of intimal thickening formation in P2Y<sub>2<\/sub>R<sup>-\/-<\/sup> mice (n = 15) 14 days after cuff placement. In vitro, loss of P2Y<sub>2<\/sub>R significantly impaired monocyte migration in response to nucleotide agonists. Furthermore, transgenic rats overexpressing the P2Y<sub>2<\/sub>R developed accelerated intimal lesions resulting in more than 95% luminal stenosis (p < 0.05, n = 10). <b><i>Conclusions:<\/i><\/b> Loss- and gain-of-function approaches established direct evidence for P2Y<sub>2<\/sub>R involvement in neointimal hyperplasia. Specific anti-P2Y<sub>2<\/sub>R therapies may be used against restenosis and bypass graft failure.<\/jats:p>","DOI":"10.1159\/000449059","type":"journal-article","created":{"date-parts":[[2016,10,10]],"date-time":"2016-10-10T21:01:52Z","timestamp":1476133312000},"page":"163-171","source":"Crossref","is-referenced-by-count":16,"title":["Direct Evidence for P2Y<sub>2<\/sub> Receptor Involvement in Vascular Response to Injury"],"prefix":"10.1159","volume":"53","author":[{"given":"Yuksel","family":"Agca","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaomin","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cansu","family":"Agca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheikh I.","family":"Seye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,10,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/362801a0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.3.445"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<300::AID-PATH596>3.0.CO;2-I"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.18.6.915"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.6.1121"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.17.3.490"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.102.14.1697"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24348"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.65.3.531"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/BF01741386"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041530202"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(95)90178-7"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199601)166:1<57::AID-JCP7>3.0.CO;2-F"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1161\/hh2101.098617"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000013700.98464.8E"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-009-9158-y"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000038111.00518.35"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1159\/000194274"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.17.12.3602"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102568200"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.313189"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2016.06.001"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1161\/hc5001.100663"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-10-0886"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401799200"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00344.2005"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.16.1.28"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.162271"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.002642"}],"container-title":["Journal of Vascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449059","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449059","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:09:20Z","timestamp":1745428160000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000449059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":29,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2016,12,13]]},"published-print":{"date-parts":[[2016,12,15]]}},"URL":"https:\/\/doi.org\/10.1159\/000449059","archive":["Portico"],"relation":{},"ISSN":["1018-1172","1423-0135"],"issn-type":[{"value":"1018-1172","type":"print"},{"value":"1423-0135","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}