{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:40:11Z","timestamp":1745430011500,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T00:00:00Z","timestamp":1478217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T00:00:00Z","timestamp":1478217600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfus Med Hemother"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>&lt;b&gt;Background: &lt;\/b&gt;The HCV RNA testing of potential cornea donors frequently relies on blood samples stored pre mortem. The recommended storage time of maximum 72 h frequently excludes a significant fraction of donors. &lt;b&gt;Methods: &lt;\/b&gt;The influence of storage time of EDTA plasma samples at 4 \u00b0C on the viral load measured with the Roche HCV Quantitative Test vs. 2.0 was evaluated for 43 samples from HCV-positive individuals. &lt;b&gt;Results:&lt;\/b&gt; The mean reduction of the viral load after 4 \u00b0C storage for 6-8 days was 0.46 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml (range +0.17 to -1.66 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml). After 1-3 days a mean loss of 0.19 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml (range +0.30 to -1.41 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml) and after 3-5 days of 0.32 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml (range +0.36 to -1.81 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml) was observed. In 23.3% of samples, a viral load reduction \u2265 1 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml (1.0-1.81 log&lt;sub&gt;10&lt;\/sub&gt; IU\/ml) was found after prolonged storage (5-8 days). In none of the samples did the HCV load fall below the detection limit. &lt;b&gt;Conclusion:&lt;\/b&gt; Plasma storage for up to 8 days can quantitatively reduce the HCV RNA load, yet has no influence on the reliability of a qualitative HCV RNA detection by this ultrasensitive test to determine the HCV status of serologically negative cornea donors.<\/jats:p>","DOI":"10.1159\/000449207","type":"journal-article","created":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T22:01:30Z","timestamp":1478210490000},"page":"39-44","source":"Crossref","is-referenced-by-count":3,"title":["HCV RNA Testing of Plasma Samples from Cornea Donors: Suitability of Plasma Samples Stored at 4 \u00b0C for up to 8 Days"],"prefix":"10.1159","volume":"44","author":[{"given":"Annemarie","family":"Berger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Salla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver T.","family":"Keppler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger F.","family":"Rabenau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,11,4]]},"container-title":["Transfusion Medicine and Hemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:10:23Z","timestamp":1745428223000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000449207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,4]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,1,27]]}},"URL":"https:\/\/doi.org\/10.1159\/000449207","archive":["Portico"],"relation":{},"ISSN":["1660-3796","1660-3818"],"issn-type":[{"type":"print","value":"1660-3796"},{"type":"electronic","value":"1660-3818"}],"subject":[],"published":{"date-parts":[[2016,11,4]]}}}