{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T17:17:31Z","timestamp":1776964651772,"version":"3.51.4"},"reference-count":13,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2016,10,29]],"date-time":"2016-10-29T00:00:00Z","timestamp":1477699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,10,29]],"date-time":"2016-10-29T00:00:00Z","timestamp":1477699200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2017]]},"abstract":"<b><i>Background:<\/i><\/b> Topical antimicrobial formulations containing neomycin are commonly used to prevent and treat burn infections. However, <i>Pseudomonas aeruginosa<\/i> shows rapid acquisition of adaptive resistance to neomycin. This study aimed to evaluate the survival of <i>P. aeruginosa<\/i> during exposure to neomycin at high concentrations comparable to those used in topical formulations, and to investigate the effect of adaptive resistance to neomycin on the susceptibility to other aminoglycosides. <b><i>Methods:<\/i><\/b> Strain IID1130 [neomycin minimal inhibitory concentration (MIC) = 4 \u00b5g\/ml] was incubated on an agar medium containing neomycin at high concentrations (8-4,096 \u00b5g\/ml), and growing colonies were macroscopically observed. Acquisition of adaptive resistance was examined for 5 <i>P. aeruginosa<\/i> strains. Cells were sequentially passaged on agar medium containing neomycin with step-wise increased concentrations (8-2,048 \u00b5g\/ml). To assess reversion of antibiotic susceptibility, the resulting colonies were repeatedly subcultured on antibiotic-free agar plates. <b><i>Results:<\/i><\/b> Growing IID1130 colonies were macroscopically detected on a neomycin-containing (2,048 \u00b5g\/ml) agar plate for 48 h. These cells showed increasing MIC for not only neomycin, but also gentamicin and amikacin; the MIC values were occasionally higher than the breakpoints. When the adapted cells were subcultured on antibiotic-free agar, several passages were required for reversion of susceptibility. <b><i>Conclusions:<\/i><\/b> Our findings suggest that <i>P. aeruginosa<\/i> can survive in the presence of neomycin with a concentration typically used in topical dosage forms, and that the acquired adaptive resistance is persistent and is accompanied by cross-resistance to other aminoglycosides.<\/jats:p>","DOI":"10.1159\/000449368","type":"journal-article","created":{"date-parts":[[2016,10,31]],"date-time":"2016-10-31T01:26:46Z","timestamp":1477877206000},"page":"121-127","source":"Crossref","is-referenced-by-count":6,"title":["Adaptive Cross-Resistance to Aminoglycoside Antibiotics in <b><i>Pseudomonas aeruginosa<\/i><\/b> Induced by Topical Dosage of Neomycin"],"prefix":"10.1159","volume":"62","author":[{"given":"Shuji","family":"Uemura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-ichi","family":"Yokota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsukasa","family":"Shiraishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Kitagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suguru","family":"Hirayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoko","family":"Kyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotoshi","family":"Mizuno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keigo","family":"Sawamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Miyamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eichi","family":"Narimatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2016,10,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.2174\/1574891X08666131112143447"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/00004630-199709000-00006"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.08.009"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000437252"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.11.019"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.2165\/11597020-000000000-00000"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0223"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0b013e3181921eed"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.2.414"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.36.9.1951"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.4.1371-1375.2003"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04099-14"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-29-1-41"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449368","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449368","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/449368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:11:10Z","timestamp":1745428270000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000449368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,29]]},"references-count":13,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2,15]]}},"URL":"https:\/\/doi.org\/10.1159\/000449368","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"value":"0009-3157","type":"print"},{"value":"1421-9794","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,29]]}}}