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Karger AG","issue":"2","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2017]]},"abstract":"To more efficiently process the large sample numbers for quantitative determination of ammonium (NH<sub>4<\/sub><sup>+<\/sup>) and phosphate (orthophosphate, PO<sub>4<\/sub><sup>3-<\/sup>) generated during comprehensive growth experiments with the marine <i>Roseobacter<\/i> group member<i> Phaeobacter inhibens<\/i> DSM 17395, specific colorimetric assays employing a microplate reader (MPR) were established. The NH<sub>4<\/sub><sup>+<\/sup> assay is based on the reaction of NH<sub>4<\/sub><sup>+<\/sup> with hypochlorite and salicylate, yielding a limit of detection of 14 \u00b5<smlcap>M<\/smlcap>, a limit of quantitation of 36 \u00b5<smlcap>M,<\/smlcap> and a linear range for quantitative determination up to 200 \u00b5<smlcap>M<\/smlcap>. The PO<sub>4<\/sub><sup>3-<\/sup>assay is based on the complex formation of PO<sub>4<\/sub><sup>3-<\/sup> with ammonium molybdate in the presence of ascorbate and zinc acetate, yielding a limit of detection of 13 \u00b5<smlcap>M<\/smlcap>, a limit of quantitation of 50 \u00b5<smlcap>M,<\/smlcap> and a linear range for quantitative determination up to 1 m<smlcap>M<\/smlcap>. Both MPR-based assays allowed for fast (significantly lower than 1 h) analysis of 21 samples plus standards for calibration (all measured in triplicates) and showed only low variation across a large collection of biological samples.<\/jats:p>","DOI":"10.1159\/000454814","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T12:59:55Z","timestamp":1486558795000},"page":"73-80","source":"Crossref","is-referenced-by-count":8,"title":["Photometric Determination of Ammonium and Phosphate in Seawater Medium Using a Microplate Reader"],"prefix":"10.1159","volume":"27","author":[{"given":"Hanna S.","family":"Ruppersberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maren R.","family":"Goebel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svea 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