{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T10:05:26Z","timestamp":1775556326531,"version":"3.50.1"},"reference-count":38,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Caries Res"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Although several studies have demonstrated the efficacy of AmF\/NaF\/SnCl&lt;sub&gt;2&lt;\/sub&gt; solution in inhibiting dental erosion progression, measures for further improvement in its effectiveness are paramount. Thus, this in situ study evaluated whether the protective effect promoted by the AmF\/NaF\/SnCl&lt;sub&gt;2&lt;\/sub&gt; solution would be enhanced by increasing its frequency of use. The study was conducted with 12 volunteers, a 4-phase (5 days each) randomized, crossover model. Extraoral erosive challenges (0.5% citric acid, pH 2.6, 6 \u00d7 2 min\/day) and rinsing protocol (1 or 2 \u00d7 2 min\/day) were performed. Before the in situ phase, human enamel samples were subjected to an in vitro surface softening (1% citric acid, pH 4.0, for 3 min). Four treatment protocols were tested using samples in replicas (&lt;i&gt;n&lt;\/i&gt; = 12): group G1 - deionized water (negative control); G2 - NaF solution (positive control, 500 ppm F&lt;sup&gt;-&lt;\/sup&gt;, pH 4.5); G3 - AmF\/NaF\/SnCl&lt;sub&gt;2&lt;\/sub&gt; solution (500 ppm F&lt;sup&gt;-&lt;\/sup&gt;, 800 ppm Sn&lt;sup&gt;2+&lt;\/sup&gt;, pH 4.5) once a day; G4 - AmF\/NaF\/SnCl&lt;sub&gt;2&lt;\/sub&gt; solution twice a day. Tissue loss and morphological changes were determined by optical profilometry (&lt;i&gt;n&lt;\/i&gt; = 12) and scanning electron microscopy (&lt;i&gt;n&lt;\/i&gt; = 3) analysis, respectively. Data were statistically analyzed by ANOVA with subsequent pairwise comparison of treatments. Tissue loss means (\u00b1SD in \u00b5m) for each treatment protocol and statistical differences were found as follows: G1 4.55 \u00b1 2.75, G2 4.59 \u00b1 2.13, G3 2.64 \u00b1 1.55, and G4 1.34 \u00b1 1.16. Although there was no difference between the 2 AmF\/NaF\/SnCl&lt;sub&gt;2&lt;\/sub&gt; solution application regimens (once or twice a day), application of the product twice a day was the only treatment that was able to control erosion progression, differing from the control groups.<\/jats:p>","DOI":"10.1159\/000455051","type":"journal-article","created":{"date-parts":[[2017,1,26]],"date-time":"2017-01-26T22:16:55Z","timestamp":1485469015000},"page":"141-148","source":"Crossref","is-referenced-by-count":17,"title":["Frequency of Application of AmF\/NaF\/SnCl&lt;sub&gt;2&lt;\/sub&gt; Solution and Its Potential in Controlling Human Enamel Erosion Progression: An in situ Study"],"prefix":"10.1159","volume":"51","author":[{"given":"C.V.","family":"da Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.M.","family":"Ramos-Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.F.","family":"Mantilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.M.","family":"de 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