{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T03:37:51Z","timestamp":1768275471406,"version":"3.49.0"},"reference-count":18,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Mantle cell lymphoma (MCL) remains incurable and new treatments are needed, especially in the relapsed\/refractory setting. We therefore investigated the effects of carfilzomib, a novel, long-acting, second-generation proteasome inhibitor, in MCL cells. Eight established MCL cell lines and freshly isolated primary MCL cells were treated with carfilzomib. Cell proliferation was assessed by a &lt;sup&gt;3&lt;\/sup&gt;H-thymidine incorporation assay. Cell apoptosis was evaluated by flow cytometry with annexin V and propidium iodide. Electrophoresis mobility shift (EMSA), Western blot, and luciferase assays were used to analyze NF-&amp;#x03BA;B activation and related signaling proteins. Carfilzomib inhibited growth and induced apoptosis in both established MCL cell lines and freshly isolated primary MCL cells in a dose-dependent manner. In contrast, carfilzomib was less toxic to normal peripheral blood mononuclear cells from healthy individuals. The carfilzomib-induced apoptosis of MCL cells occurred in a caspase-dependent manner through both intrinsic and extrinsic caspase pathways. In addition, carfilzomib inhibited constitutive activation of the NF-&amp;#x03BA;B signaling cascade, both in MCL cell lines and primary MCL cells, by completely blocking the phosphorylation of I&amp;#x03BA;B\u03b1. Our results demonstrate that carfilzomib can induce growth arrest and apoptosis in MCL cells and that the mechanism may involve the NF-&amp;#x03BA;B signaling pathway.<\/jats:p>","DOI":"10.1159\/000455939","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T22:03:25Z","timestamp":1487282605000},"page":"106-112","source":"Crossref","is-referenced-by-count":13,"title":["Carfilzomib Inhibits Constitutive NF-&amp;#x03BA;B Activation in Mantle Cell Lymphoma B Cells and Leads to the Induction of Apoptosis"],"prefix":"10.1159","volume":"137","author":[{"given":"Yong-Li","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew Ho Zhi","family":"Guang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Qin","family":"Zhuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang-Hui","family":"Min","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai-Qi","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng-Hua","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dai-Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Yuan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Han","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-Jie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,2,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.16.8435"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdn045"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050147"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/psc.1107"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2012.09.002"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2005.01570.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.1.88"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-4086"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302559200"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.22.9695-9704.2004"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200360200"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043445"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-065888"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2012.08.003"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/nrc906"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100119497"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1126\/science.3140380"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(01)01446-8"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/455939","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/455939","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/455939","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:12:00Z","timestamp":1745428320000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000455939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":18,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,3,8]]}},"URL":"https:\/\/doi.org\/10.1159\/000455939","archive":["Portico"],"relation":{},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"value":"0001-5792","type":"print"},{"value":"1421-9662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}