{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T09:34:55Z","timestamp":1776850495613,"version":"3.51.2"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dev Pharmacol Ther"],"abstract":"<jats:p>Albumin was isolated from pooled fetal serum obtained at normal delivery at\nterm and from pooled adult plasma. Albumin isolation was carried out by means of PEG\nprecipitation followed by ion exchange chromatography on DEAE-Sephadex A 50 and then\non SP-Sephadex C 50. The binding of diazepam (1 \u03bcM), salicylic acid (2 mM) and digitoxin\n(6 nM) to albumin (40 g\/l) was measured by equilibrium dialysis at 37\u00b0C. The unbound\nfraction (mean \u00b1 SD) for fetal and adult albumin of diazepam was 1.86 \u00b1 0.24 and 1.82 \u00b1\n0.15% (NS), that of digitoxin was 3.18 \u00b1 0.27 and 3.36 \u00b1 0.04% (NS) and that of salicylic\nacid was 11.65 \u00b1 0.99 and 9.47 \u00b1 0.75% (p &lt; 0.05), respectively. With both fetal and adult\nalbumin, a single class of binding sites was observed for diazepam and digitoxin, whereas two\nclasses of binding sites were observed for salicylic acid. The number of binding sites (n,\nmoles of drug per mole of albumin) for fetal and adult albumin was 0.83 and 1.02 for\ndiazepam and 0.014 and 0.018 for digitoxin, respectively. For salicylic acid, n was 1.45 (fetal\nalbumin) and 1.55 (adult albumin) for the higher affinity site, and 3.06 (fetal albumin) and\n3.27 (adult albumin) for the lower affinity site. The association constant (K(a), M^-1) for diazepam\nwas 1.36 \u00d7 10^5 (fetal albumin) and 1.00 \u00d7 10^5 (adult albumin) and that for digitoxin\nwas 4.12 \u00d7 10^6 (fetal albumin) and 2.7 \u00d7 10^6 (adult albumin). For salicylic acid, K(a) was 38.4\n\u00d7 10^3 (fetal albumin) and 35.8 \u00d7 10^3 (adult albumin) for the higher affinity site, and 2.7 \u00d7\n10^3 (fetal albumin) and 4.3 \u00d7 10^3 (adult albumin) for the lower affinity site. This work shows\nthat fetal and adult albumin have similar binding properties and corroborates our previous\nfindings with furosemide.<\/jats:p>","DOI":"10.1159\/000457505","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:08:31Z","timestamp":1502471311000},"page":"100-108","source":"Crossref","is-referenced-by-count":11,"title":["Binding of Diazepam, Salicylic Acid and Digitoxin to Albumin\nIsolated from Fetal and Adult Serum"],"prefix":"10.1159","author":[{"given":"Antonella","family":"Viani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Cappiello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Maria","family":"Pacifici","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Developmental Pharmacology and Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000457505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T08:34:41Z","timestamp":1776846881000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000457505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000457505","archive":["Portico"],"relation":{},"ISSN":["0379-8305","2504-2505"],"issn-type":[{"value":"0379-8305","type":"print"},{"value":"2504-2505","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}