{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:26:05Z","timestamp":1777868765080,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>The biochemical triad, NADH oxidation, oxygen (0(2)) uptake and hydrogen peroxide\n(H(2)0(2)) formation, by subcellular fractions of human blood polymorphonuclears (PMNs) was\ninvestigated. It was found that this biochemical triad (1) was under the control of the granule-rich\nfraction (GRF) only; (2) was not inhibited by cyanide; (3) occurred stoichiometrically for its three\ncomponents, and (4) accounted quantitatively for the respiratory burst of the stimulated PMN.\nIt was also shown that the above biochemical triad (1) involved an enzymatic step; (2) was\nenhanced by acidic pH (5.0) and Mg^++; (3) was inhibited by Cu^++ or low concentration of Mn^++;\n(4) was dependent on H(2)0(2), perhydroxyl radical (HO(2)) and hydroxyl radical (HO\u2022) since either\ncatalase or superoxide dismutase or scavengers of HO\u2022(2) or HO. were inhibitor, and (5) involved\nmultistep reactions. Evidence is provided that the sequence of the reactions is first a generation of\nH(2)0(2) (spontaneously from NADH in our incubation medium), secondly the production of HO\u2022\nfrom H(2)0(2), thirdly the oxidation of NADH with further production of HO\u2022(2), 02 uptake and H(2)0(2)\nformation, probably through a chain reaction.\nThe identification of the enzyme(s) involved in these multistep reactions needs further studies.<\/jats:p>","DOI":"10.1159\/000458584","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:10:50Z","timestamp":1502471450000},"page":"225-237","source":"Crossref","is-referenced-by-count":5,"title":["Cyanide-Insensitive NADH Oxidation\nby Subcellular Fractions Isolated from Human\nPolymorphonuclear Blood Cells"],"prefix":"10.1159","author":[{"given":"Christian","family":"Auclair","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Cramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Hakim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000458584","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:39:23Z","timestamp":1777552763000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000458584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000458584","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}