{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:26:12Z","timestamp":1777868772286,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>A sensitive and precise assay of guanase was based on the conversion of 14C-guanine\nto uric acid in the presence of excess xanthine oxidase. The enzyme was entirely soluble in rat\ntissues and no inhibitors of it were detected. The most active tissues were red blood cells, lung and\nlactating mammary gland, with more than twice the activity of liver. The contained blood could\naccount for the low activity in adult skeletal muscle and some other tissues. All fetal tissues\nexamined were without activity.\nActivity in mammary gland rose fourfold during lactation and dropped precipitously during\ninvolution, with a secondary rise associated in time with the loss of cells from the gland.\nReticulocytes present normally and after hemorrhagic anemia appeared to account for substantially\nall of the high guanase activity in red blood cells. The guanase level could be used to predict\nthe degree of reticulocytosis in rats within confidence limits of \u00b1 0.2%. The virtual absence of\nguanase in human red cells was confirmed, even in bloods containing elevated reticulocyte\nnumbers.<\/jats:p>","DOI":"10.1159\/000458608","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:10:36Z","timestamp":1502471436000},"page":"395-403","source":"Crossref","is-referenced-by-count":4,"title":["Guanase in Reticulocytes and Other Rat Tissues"],"prefix":"10.1159","author":[{"given":"Hal B.","family":"Hindman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Eugene","family":"Knox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000458608","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:39:31Z","timestamp":1777552771000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000458608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000458608","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}