{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:26:20Z","timestamp":1777868780381,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>Human placental alkaline phosphatase\n(AP) exhibits a genetic polymorphism\nthat is determined by the genotype of the fetus.\nA similar enzyme is produced by certain neoplasms\nand by some cells in culture. The relationships\nbetween these enzymes were investigated\nby immunological methods comparing human\nplacental AP to the AP of HeLa(71) cells. Antiseium\nprepared against placental AP precipitated 90% of the enzyme and the catalytic\nactivity was quantitatively recovered in the antigen-antibody precipitate. Enzyme-antibody\ncomplexes failed to migrate on starch gel electrophoresis. Antiserum against placental AP\ncross-reacted with HeLa(71) AP and HeLa(71) AP antiserum reacted with the placental enzyme.\nImmunological analysis by double diffusion in agar showed that the three common genetic\nvariants of placental AP - F, FS and S - and the HeLa(71) AP were closely related when\nstudied by antisera against both placental or HeLan AP. When studied by immunodiffusion\nthe genetic variants of human placental AP and the HeLa enzyme reacted with identity at\nthe points of contact of the precipitation lines when precipitated by antisera against either\nenzyme. These findings support the view that the human placental and HeLa(71) APs are\nproducts of the same genetic locus. Derepression of a portion of the genome in association\nwith malignant transformation might be responsible for ectopic production of this enzyme\nin HeLa(71) cells.<\/jats:p>","DOI":"10.1159\/000458917","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:11:25Z","timestamp":1502471485000},"page":"35-45","source":"Crossref","is-referenced-by-count":8,"title":["Immunological Characterization of Oncofetal Alkaline\nPhosphatases from Human Placenta and HeLa(71) Cells"],"prefix":"10.1159","author":[{"given":"Nimae K.","family":"Ghosh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rody P.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000458917","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:40:50Z","timestamp":1777552850000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000458917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000458917","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}