{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:26:33Z","timestamp":1777868793815,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>Using agarose gel electrophoresis, a faster moving alanine aminopeptidase\n(EC 3.4.11.2) has been demonstrated in the urine from cases of Fanconi syndrome, endemic\n(Balkan) nephropathy and advanced renal insufficiency. The enzyme was partially purified\nand its properties (isoelectric point, molecular weight, substrate specificity, influence of metal\nions, Michaelis constant, antigenic behavior) were compared with those of normal kidney\nalanine aminopeptidase. Isoelectric points and antigenic properties are identical, but the\nmolecular weights differ by a factor of about 2. Therefore, the greater electrophoretic mobility\nis due to the smaller size of the atypical enzyme.<\/jats:p>","DOI":"10.1159\/000459122","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:12:23Z","timestamp":1502471543000},"page":"348-354","source":"Crossref","is-referenced-by-count":1,"title":["A Low Molecular Weight Alanine Aminopeptidase in Urine"],"prefix":"10.1159","author":[{"given":"L.","family":"Neef","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. J","family":"H\u00fctter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"B\u00f6hme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Dotschev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. T.","family":"Danev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Haschen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000459122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:42:17Z","timestamp":1777552937000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000459122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000459122","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}