{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:57:42Z","timestamp":1760122662194,"version":"3.40.4"},"reference-count":17,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2017]]},"abstract":"To investigate the immunoprotective effects of the recombinant type A flagellin (FLA), the <i>fla<\/i>A gene of <i>Legionella pneumophila<\/i> serogroup 1 strain Paris was cloned into pET28a(+). Recombinant FLA (rFLA) was overexpressed in <i>E. coli<\/i> BL21 (DE3) and purified by Ni<sup>2+<\/sup> exchange chromatography. Female BALB\/c aged 6-8 weeks were immunized with 20 \u03bcg of rFLA. Nonimmunized mice along with mice inoculated with a sublethal dose of live <i>L. pneumophila<\/i> intravenously were considered as negative and positive controls, respectively. A significant serum antibody response was observed in female BALB\/c mice immunized with rFLA. Production of IFN-&#x03B3; and IL-12, and TNF-\u03b1 in the serum and the splenocyte cultures, and antigen-specific splenocyte proliferation suggested a strong innate and adaptive cell-mediated immunity response in rFLA-immunized mice. Intravenous lethal challenge with <i>L. pneumophila<\/i> serogroup 1 (strain Paris) showed that 60% of mice immunized with rFLA survived in a 10-day follow-up survey. These results show that rFLA from <i>L. pneumophila<\/i> can elicit strong innate and adaptive immune responses and suggest the possibility of a long-term immunity against lethal challenge with <i>L. pneumophila<\/i>.<\/jats:p>","DOI":"10.1159\/000460295","type":"journal-article","created":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T21:01:56Z","timestamp":1493154116000},"page":"110-116","source":"Crossref","is-referenced-by-count":3,"title":["Protection against Legionnaire's Disease: Recombinant Flagellin A of <b><i>Legionella pneumophila<\/i><\/b> Can Induce Protective Immunity against Bacteremia in a BALB\/c Murine Model"],"prefix":"10.1159","volume":"27","author":[{"given":"Ashraf","family":"Mohabati Mobarez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roya","family":"Ahmadrajabi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nima","family":"Khoramabadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali-Hatef","family":"Salmanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,4,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-013-9696-y"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-006-6391-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113962"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114779"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-3109(00)00235-6"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.3201\/eid1609.100248"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2116-2122.2001"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.506-526.2002"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.05.004"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737591"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.001021"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5234-5240.2000"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-8-1955"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.05.013"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1086\/516530"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.02.013"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.03.010"}],"container-title":["Microbial Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/460295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/460295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:14:42Z","timestamp":1745428482000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000460295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":17,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,5,16]]}},"URL":"https:\/\/doi.org\/10.1159\/000460295","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.4906790","asserted-by":"subject"}]},"ISSN":["2673-1665","2673-1673"],"issn-type":[{"type":"print","value":"2673-1665"},{"type":"electronic","value":"2673-1673"}],"subject":[],"published":{"date-parts":[[2017]]}}}