{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T17:22:25Z","timestamp":1763140945350,"version":"3.40.4"},"reference-count":31,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>An endo-polygalacturonase secreted by &lt;i&gt;Aspergillus sojae &lt;\/i&gt;was characterized after being purified to homogeneity from submerged cultures with orange peel as the sole carbon source by gel filtration and ion-exchange chromatographies. According to SDS-PAGE and analytical isoelectric focusing analyses, the enzyme presents a molecular weight of 47 kDa and pI value of 4.2. This enzyme exhibits considerable stability under highly acidic to neutral conditions (pH 1.5-6.5) and presents a half-life of 2 h at 50\u00b0C. Besides its activity towards pectin and polygalacturonic acid, the enzyme displays pectin-releasing activity, acting best in a pH range of 3.3-5.0. Thin-layer chromatographic analysis revealed that tri-galacturonate is the main enzymatic end product of polygalacturonic acid hydrolysis, indicating that it is an endo-polygalacturonase. The enzyme exhibits Michaelis-Menten kinetics, with K&lt;sub&gt;M&lt;\/sub&gt; and V&lt;sub&gt;MAX&lt;\/sub&gt; values of 0.134 mg\/mL and 9.6 \u00b5mol\/mg\/min, respectively, and remained stable and active in the presence of SO&lt;sub&gt;2&lt;\/sub&gt;, ethanol, and various cations assayed except Hg&lt;sup&gt;2+&lt;\/sup&gt;.<\/jats:p>","DOI":"10.1159\/000460296","type":"journal-article","created":{"date-parts":[[2017,4,27]],"date-time":"2017-04-27T21:03:18Z","timestamp":1493326998000},"page":"102-109","source":"Crossref","is-referenced-by-count":6,"title":["Purification and Biochemical and Kinetic Properties of an Endo-Polygalacturonase from the Industrial Fungus &lt;b&gt;&lt;i&gt;Aspergillus sojae&lt;\/i&gt;&lt;\/b&gt;"],"prefix":"10.1159","volume":"27","author":[{"given":"Dante","family":"Fratebianchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana Alejandra","family":"Cavello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n Fernando","family":"Cavalitto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,4,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00080.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.12.030"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018427614046"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008917912296"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(99)80174-1"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.01.010"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2007.06.005"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1042\/bj0550170"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2007.08.008"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2007.03.010"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.5006"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb08946.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s002530050951"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2006.09.023"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016102522747"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.02.016"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2007.10.002"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2005.03.026"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-89132004000500004"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-006-9114-6"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2015.12.005"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2006.06.009"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150090603"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.01959.x"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.201300651"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-010-0702-0"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023959727067"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2007.05.025"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.04.056"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1006\/fstl.1993.1023"}],"container-title":["Microbial Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/460296","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/460296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:14:43Z","timestamp":1745428483000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000460296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":31,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,5,16]]}},"URL":"https:\/\/doi.org\/10.1159\/000460296","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.4929530","asserted-by":"subject"}]},"ISSN":["2673-1665","2673-1673"],"issn-type":[{"type":"print","value":"2673-1665"},{"type":"electronic","value":"2673-1673"}],"subject":[],"published":{"date-parts":[[2017]]}}}