{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:39:05Z","timestamp":1792701545640,"version":"4.5.0"},"reference-count":42,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Adult stem cells have been studied as a promising therapeutic modality for the functional restoration of the damaged heart. In the present study, a strategy for enhancing the angiogenic efficacy of human mesenchymal stem cells (hMSCs) using micro-RNA was examined. We investigated whether micro-RNA-146a (miR-146a) influences the secretion of vascular endothelial growth factor (VEGF) and angiogenesis of MSCs. Our data indicated that miR-146a-transfected hMSCs (hMSC&lt;sup&gt;miR-146a&lt;\/sup&gt;) decreased the expression of neurofibromin 2, an inhibitor of p21-activated kinase-1 (PAK1). miR-146a also increased the expression of Ras-related C3 botulinum toxin substrate 1 and PAK1, which are known to induce VEGF expression, and the formation of vascular branches was increased in hMSC&lt;sup&gt;miR-146a&lt;\/sup&gt; compared to hMSCs treated with VEGF. VEGF and p-Akt were increased in hMSC&lt;sup&gt;miR-146a&lt;\/sup&gt;. Furthermore, injection of hMSC&lt;sup&gt;miR-146a&lt;\/sup&gt; after ischemia\/reperfusion (I\/R) injury led to a reduction of fibrosis area and increased VEGF expression, confirming the regenerative capacity such as reparative angiogenesis in the infarcted area. Cardiac functions in I\/R injury were improved following injection of hMSC&lt;sup&gt;miR-146a&lt;\/sup&gt; compared to the I\/R group. Taken together, these data suggest that miR-146 is a novel microRNA that regulates VEGF expression, and its use may be an effective strategy for enhancing the therapeutic efficacy of hMSC transplantation into the I\/R-injured heart.<\/jats:p>","DOI":"10.1159\/000461596","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T17:01:57Z","timestamp":1492102917000},"page":"100-108","source":"Crossref","is-referenced-by-count":57,"title":["Exogenous miRNA-146a Enhances the Therapeutic Efficacy of Human Mesenchymal Stem Cells by Increasing Vascular Endothelial Growth Factor Secretion in the Ischemia\/Reperfusion-Injured Heart"],"prefix":"10.1159","volume":"54","author":[{"given":"Hyang-Hee","family":"Seo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se-Yeon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang Youn","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilseog","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sekyung","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hojin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Young","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jongmin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lark Kyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki-Chul","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woochul","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2017,4,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.128413"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2012.04.002"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s11936-014-0323-4"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.211"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1517\/14712590903455997"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2014.07.003"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.2217\/rme.09.74"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00565.2008"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1160\/TH05-03-0218"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-398459-3.00015-0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000260272.21605.c8"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-398459-3.00013-7"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.07.023"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.192872"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2009.56"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.889048"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000116"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.280529"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.21.3.469"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1038\/363515a0"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-5-69"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1339"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1101\/gad.210773.112"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2012.04.006"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1097\/00130404-200401000-00006"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00382-4"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006150200"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1186\/bcr961"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.188"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20558"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.34.21512"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000110502.10593.06"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22993"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1159\/000088478"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1593\/neo.121422"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.17.9624"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1038\/86507"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000074870.54671.98"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1593\/neo.111600"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.3013"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1101\/gad.924501"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1186\/ar3798"}],"container-title":["Journal of Vascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/461596","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/461596","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/461596","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T13:16:45Z","timestamp":1745414205000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000461596"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":42,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,5,5]]}},"URL":"https:\/\/doi.org\/10.1159\/000461596","archive":["Portico"],"relation":{},"ISSN":["1018-1172","1423-0135"],"issn-type":[{"value":"1018-1172","type":"print"},{"value":"1423-0135","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}