{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T04:44:02Z","timestamp":1788410642672,"version":"build-2803163510"},"reference-count":0,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells Tissues Organs"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Sonic Hedgehog (Shh) signaling plays a major role in and is essential for regulation, patterning, and proliferation during renal development. Smoothened (&lt;i&gt;Smo&lt;\/i&gt;) plays a pivot role in transducing the Shh-glioma-associated oncogene Kruppel family member. However, the cellular and molecular mechanism underlying the role of sustained &lt;i&gt;Smo&lt;\/i&gt; activation in postnatal kidney development is still not clearly understood. Using a conditional knockin mouse model that expresses a constitutively activated form of &lt;i&gt;Smo&lt;\/i&gt; (SmoM2) upon Homeobox-B7-mediated recombination (&lt;i&gt;Hoxb7-Cre&lt;\/i&gt;), the effects of Shh signaling were determined in postnatal kidney development. SmoM2;&lt;i&gt;Hoxb7-Cre&lt;\/i&gt; mutant mice showed growth retardation with a reduction of body weight. Constitutive activation of &lt;i&gt;Smo&lt;\/i&gt; in the renal collecting ducts caused renal hypoplasia, hydronephrosis, and hydroureter. The parenchymal area and glomerular numbers were reduced, but the glomerular density was increased in SmoM2;&lt;i&gt;Hoxb7-Cre&lt;\/i&gt; mutant mice. The expression of Patched 1, the receptor of Shh and a downstream target gene of the Shh signaling pathway, was highly restricted and it was upregulated in the inner medullary collecting ducts of the kidney. The proliferative cells in the mesenchyme and collecting ducts were decreased in SmoM2;&lt;i&gt;Hoxb7-Cre&lt;\/i&gt; mutant mice. This study showed for the first time that sustained &lt;i&gt;Smo&lt;\/i&gt; inhibits postnatal kidney development by suppressing the proliferation of the mesenchyme and medullary collecting ducts in mice.<\/jats:p>","DOI":"10.1159\/000464460","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T21:02:47Z","timestamp":1496264567000},"page":"38-48","source":"Crossref","is-referenced-by-count":5,"title":["Constitutive Activation of Smoothened in the Renal Collecting Ducts Leads to Renal Hypoplasia, Hydronephrosis, and Hydroureter"],"prefix":"10.1159","volume":"204","author":[{"given":"Deepak Prasad","family":"Gupta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Won","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui-Sic","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang Ho","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ok Hee","family":"Chai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2017,6,1]]},"container-title":["Cells Tissues Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/464460","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/464460","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:33:58Z","timestamp":1745429638000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000464460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,7,6]]}},"URL":"https:\/\/doi.org\/10.1159\/000464460","archive":["Portico"],"relation":{},"ISSN":["1422-6405","1422-6421"],"issn-type":[{"value":"1422-6405","type":"print"},{"value":"1422-6421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}