{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:20:34Z","timestamp":1777868434381,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme Protein"],"abstract":"<jats:p>Alkaline phosphatase solubilized from a human Hodgkin\u2019s\nlymphoma cell line (L428) was compared with purified amphiphilic\nand hydrophilic forms of the enzyme from human liver,\nand with the enzyme solubilized from a cultured osteosarcoma\ncell line (Saos-2). Purified hydrophilic alkaline phosphatases\nfrom human placenta and intestine were also compared in\nsome experiments. Alkaline phosphatase was released from the\nplasma membrane of intact lymphocytes by phosphatidylinositol\nphospholipase C and thus is anchored to the outside of the\nplasma membrane by covalently attached phosphatidylinositol.\nEnzyme released in this way was hydrophilic and that solubilized\nwith Triton X-100 was amphiphilic, as assessed by\nadsorption to octyl-Sepharose. Lymphocyte alkaline phosphatase,\nwhen released from the membrane by phosphatidylinositol\nphospholipase C or solubilized by Triton X-100, had apparent\nM(r) values on gradient gel electrophoresis of 227 and 494\nkDa, respectively. These values were consistently higher than\nequivalent ones obtained with enzymes purified from human\nliver, but were similar to those of cultured osteosarcoma cells.\nIsoenzyme-specific inhibitors of alkaline phosphatase showed\nsimilar patterns of inhibition between the enzyme from L428\ncells and the tissue-nonspecific (liver\/kidney\/bone) isoenzyme\nfrom human liver. Heat stabilities were similar for the enzymes\nfrom L428 and Saos-2 (bone isoform) cell lines, but differed\nsignificantly from those of liver, intestine and placenta. We\nconclude that the alkaline phosphatase expressed in this lymphoma\ncell line (L428) has properties that most closely resemble\nthose of the tissue-nonspecific isoenzyme found normally in\nosteoblasts of bone (bone isoform).<\/jats:p>","DOI":"10.1159\/000468660","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:20:45Z","timestamp":1502472045000},"page":"73-82","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of the Alkaline\nPhosphatase Expressed on the\nSurface of a Hodgkin's Lymphoma\nCell Line"],"prefix":"10.1159","author":[{"given":"Liane","family":"Belland","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Visser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Poppema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Stinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme and Protein"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000468660","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:56:25Z","timestamp":1777550185000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000468660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000468660","archive":["Portico"],"relation":{},"ISSN":["1019-6773","2504-2556"],"issn-type":[{"value":"1019-6773","type":"print"},{"value":"2504-2556","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}