{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:27:18Z","timestamp":1777868838392,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>A kinetic study of the inhibition of several alkaline phosphatase (AP) isoenzyme\nactivities by ph\u00e9nobarbital was carried out using p-nitrophenylphosphate ( 10 mM) as a substrate\nat pH 9.8 in a 300-mM Hepes buffer. AP from bovine kidney, calf intestine, bovine\nliver, and rat bone was used. Over a ph\u00e9nobarbital concentration range of 20-400 mM, all\nthese isoenzymes were inhibited in an uncompetitive manner with a K(1) of 200 mM for\nintestinal AP, and in a linear mixed-type manner for all the other isoenzymes tested. The K,\nvalues were 10, 40 and 55 mM for kidney, bone and liver AP. respectively. The use of 15 mM\ncarbonate-bicarbonate or 400 raM diethanolamine buffer did not modify the degree of inhibition\nof intestinal AP activity. Dixon plots of the reciprocal of reaction velocity versus\ninhibitor concentration either at different substrate concentration or at different DEA concentration\nindicate uncompetitive inhibition for the intestinal enzyme. This in vitro inhibitory\neffect of phenobarbital is in contrast to its in vivo stimulating action on AP. However, in\nthe whole animal, the effects of ph\u00e9nobarbital administration probably represent the sum of\nmultiple effects.<\/jats:p>","DOI":"10.1159\/000468804","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:21:13Z","timestamp":1502472073000},"page":"276-283","source":"Crossref","is-referenced-by-count":3,"title":["In vitro Inhibition of Alkaline Phosphatase Activities from\nIntestine, Bone, Liver, and Kidney by Ph\u00e9nobarbital"],"prefix":"10.1159","author":[{"given":"S.","family":"Tardivel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Banide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Porembska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Dupuis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Aymard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Lacour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000468804","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:45:43Z","timestamp":1777553143000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000468804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000468804","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}