{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:27:21Z","timestamp":1777868841817,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>6-Phosphofructo-1-kinase (PFK) activity in the brain of a dog affected by glycogen\nstorage disease type VII was only 31 % of the PFK activity in the normal dog brain.\nPFK in the normal dog brain was composed of L-type, M-type and C-type subunits with\napparent molecular weights of 78,000, 86,000, and 88,000, respectively, and subunit proportions\n(L:M:C) of 27:49:24. PFK in the affected dog brain was composed of nearly equal levels\nof the normal L-type and C-type subunits, but a normal M-type subunit was not detected.\nUsing antidog muscle PFK IgG, immunoblots of gels containing partially purified PFK from\nthe affected dog brain revealed a small amount of immunoreactive protein with an apparent\nmolecular weight of 84,000, suggesting the presence of a truncated M-type subunit. Kinetic\nstudies indicated that the PFK isozymes in the affected dog brain exhibited significantly\ndifferent kinetic regulatory properties when compared to the PFK isozyme pool in the normal\ndog brain.<\/jats:p>","DOI":"10.1159\/000468880","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:21:32Z","timestamp":1502472092000},"page":"137-144","source":"Crossref","is-referenced-by-count":10,"title":["Presence of a Truncated M-Type Subunit and Altered Kinetic\nProperties of 6-Phosphofructo-l-Kinase Isozymes in the Brain\nof a Dog Affected by Glycogen Storage Disease Type VII1"],"prefix":"10.1159","author":[{"given":"Yashanad","family":"Mhaskar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urs","family":"Giger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George A.","family":"Dunaway","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000468880","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:45:59Z","timestamp":1777553159000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000468880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000468880","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}