{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:27:23Z","timestamp":1777868843009,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>Photolysis of the lysosomal neuraminidase in crude homogenates of cultured\nhuman skin fibroblasts was carried out using the potent competitive enzyme inhibitor,\n9-S-(4-azido-2-nitrophenyl)-5-acetamido-2,6-anhydro-2,3,5,9-tetradeoxy-9-thio-D-glycero-\nD-galacto-non-2-enonic acid (9-PANP-2,3-D-NANA). Irradiation of the homogenate and the\ninhibitor (2 min, pH 4.3, 10 \u00b0C) with a medium pressure mercury lamp resulted in about a\n24% reduction of enzyme activity compared to irradiated controls that did not contain\nadditives. No significant loss of activity was observed with homogenate that contained a\nphotoreactive thioglycoside of sialic acid that was not an inhibitor of the enzyme. Similarly,\nthe enzyme activity was not affected when 2-deoxy-2,3-dehydro-N-acetyl neuraminic acid\nwas photolyzed with the homogenate. The latter is a potent competitive inhibitor but it is not\nphotoreactive. Also, the products obtained by prephotolyzing 9-PANP-2,3-D-NANA gave\nsimilar enzyme levels under standard assay conditions when compared with the nonirradiated\nmaterial. Together, these results demonstrate that the photoinactivation is highly\nspecific and both the aryl azide and the unsaturated pyran portion of the molecule are\nrequired for inactivation. The title compound may be useful as a potential photolabeling\nreagent which may facilitate purification of the enzyme and permit further characterization\nof the mutation in sialidosis patients.<\/jats:p>","DOI":"10.1159\/000469016","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:21:40Z","timestamp":1502472100000},"page":"103-109","source":"Crossref","is-referenced-by-count":3,"title":["Photolysis of the Lysosomal Neuraminidase in Cultured\nHuman Skin Fibroblasts in the Presence of a Photoreactive\nCompetitive Inhibitor"],"prefix":"10.1159","author":[{"given":"Thomas G.","family":"Warner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.K.","family":"Loftin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000469016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:46:16Z","timestamp":1777553176000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000469016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000469016","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}