{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:27:26Z","timestamp":1777868846095,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>The \u03b1 and \u03b1(+) isoforms of Na^+,K^+-ATPase were isolated from the kidney and\nbrain of rats and purified. Their antisera were raised to analyze the \u03b1 isoforms in rat tissues.\nWe found that the submandibular gland (SMG) contains a new immunoreactive \u03b1 subunit\nisoform, designated \u03b1(S) in this report, in addition to \u03b1 identical with those found in the\nkidney or brain. The new \u03b1(S) strongly reacted with anti-\u03b1-antiserum but to a much lesser\nextent with anti-\u03b1(+)-antiserum. The \u03b1(S) had a slightly lower molecular weight (approximately\n90,000) than the brain and kidney a isoforms. Various fractions of SMG tissues were\nadded to the SMG microsomes and incubated in order to test whether or not the \u03b1(S) is\nformed artificially; no increase of \u03b1(S) was observed by these treatments, suggesting that the\n\u03b1(S) was not the product formed from a during the preparation of microsome sample, but\nwas rather a protein originally present in the SMG.\nThe \u03b1(S) protein was not detected in the SMG of 2- or 5-week-old rats, but it gradually\nincreased in rats older than 8 weeks, reaching the maximum in 30-week-old animals. The\nNa^+,K^+-ATPase activity in the SMG increased concomitantly with the increase of \u03b1(S), indicating\nthat Na^+,K^+-ATPase comprising \u03b1(S) also shows enzyme activity; it is speculated that\n\u03b1(S) may have some unique and unknown function(s) in older rats.<\/jats:p>","DOI":"10.1159\/000469025","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:21:57Z","timestamp":1502472117000},"page":"152-159","source":"Crossref","is-referenced-by-count":1,"title":["Postnatal Changes in an \u03b1 Subunit Isoform, \u03b1(S), of\nNa^+,K^+-ATPase in the Submandibular Gland of Rats"],"prefix":"10.1159","author":[{"given":"Kazuo","family":"Hosoi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kinji","family":"Kurihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Kodama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimi","family":"Shioda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Sugita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Ueha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000469025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:46:22Z","timestamp":1777553182000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000469025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000469025","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}