{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:27:55Z","timestamp":1777868875357,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>The occurrence of alkaline phosphatase (AP) activity was examined in several\nhuman endometrial adenocarcinomas. Catalytic activities were detectable only in 10 out of 15\ntumors, with no apparent correlation between elevated AP and histological type. The apparent\nmolecular weight of the enzyme after partial purification was about 140,000 daltons.\nKinetic activity, thermodynamic properties and the pH dependence of the activity were in the\nranges reported for other subforms. Several other physicochemical properties were also investigated\nand compared with those displayed by enzymes obtained from normal human tissues.\nThe inhibition studies show that the enzyme shares several properties with the placental form,\nparticularly in resistance to zinc chloride and EDTA action. On the other hand, in sensitivity\nto uncompetitive inhibitors and to urea and ascorbic acid, it is closer to other non-Regan\nheat-sensitive forms. The results support the view that a polymorphism in the expression of\nAP in neoplastic tissues can occur. A wider spectrum of physicochemical properties is clearly\nneeded to define better the characteristics of oncodevelopmental enzymes.<\/jats:p>","DOI":"10.1159\/000469373","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:22:38Z","timestamp":1502472158000},"page":"113-121","source":"Crossref","is-referenced-by-count":3,"title":["Biochemical Properties of Alkaline Phosphatase from\nEndometrial Cancer Cells"],"prefix":"10.1159","author":[{"given":"G. A. D.","family":"Miggiano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. E.","family":"Martorana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Mordente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Castelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000469373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:48:31Z","timestamp":1777553311000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000469373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000469373","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}